Transgene expression study of CXCR4 active mutants Potential prospects in up-modulation of homing and engraftment efficiency of hematopoietic stem/progenitor cells

被引：6
作者：
Sharma, Menka [1 ]
Afrin, Farhat [2 ]
Tripathi, Rajendra P. [1 ]
Gangenahalli, Gurudutta [1 ]
机构：
[1] Inst Nucl Med & Allied Sci, Stem Cell & Gene Therapy Res Grp, Delhi, India
[2] Jamia Hamdard, Dept Biotechnol, New Delhi, India
来源：
CELL ADHESION & MIGRATION | 2014年 / 8卷 / 04期
关键词：
HSPCs; CXCR4; homing; engraftment; CHEMOKINE SDF-1; CD34(+) CELLS; NOD/SCID MICE; MIGRATION; RECEPTOR; REPOPULATION; FACTOR-1-ALPHA; LYMPHOPOIESIS; MYELOPOIESIS; ACTIVATION;
D O I：
10.4161/cam.29285
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Homing and engraftment, a determining factor in hematopoietic stem cell transplantation success is defined as a process through which hematopoietic stem/progenitor cells (HSPCs) lodge recipient bone marrow. SDF-1/CXCR4 axis acts as a principle regulator in homing and engraftment, however, CXCR4 signaling is dependent upon expression of CXCR4 and its ligand SDF-1, which is highly dynamic. Hence, present investigation was aimed to explore the potential of CXCR4 constitutive active mutants (CXCR4-CAMs) in overcoming the limitation of CXCR4 signaling and up-modulate its efficiency in homing and engraftment. Regulated transgene expression study of these mutants revealed their significantly enhanced cell adhesion efficiency to endothelium and extracellular matrix protein. This altogether indicates promising prospects of CXCR4-CAMs in research aimed to improve HSPCs engraftment efficiency.
引用
收藏
页码：384 / 388
页数：5
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