Puerarin Attenuates Osteoarthritis via Upregulating AMP-Activated Protein Kinase/Proliferator-Activated Receptor-γ Coactivator-1 Signaling Pathway in Osteoarthritis Rats

被引：54
作者：
Wang, Luyao [1 ]
Shan, Haojie [2 ]
Wang, Bin [3 ]
Wang, Nan [4 ]
Zhou, Zubin [2 ]
Pan, Chenhao [2 ]
Wang, Feng [2 ,5 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 1, Ctr Stomatol, Zhengzhou, Henan, Peoples R China
[2] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthopaed Surg, Shanghai, Peoples R China
[3] Third Peoples Hosp Haikou City, Dept Orthopaed Surg, Haikou, Hainan, Peoples R China
[4] Zhengzhou Univ, Affiliated Hosp 1, Dept Emergency, Zhengzhou, Henan, Peoples R China
[5] Shanghai Univ Med & Hlth Sci, Shanghai Peoples Hosp East 6, Dept Orthopaed Surg, Shanghai 201306, Peoples R China
来源：
PHARMACOLOGY | 2018年 / 102卷 / 3-4期
基金：
中国国家自然科学基金;
关键词：
Osteoarthritis; Rats; Puerarin; Mitochondria; AMP-activated protein kinase; MITOCHONDRIAL DYSFUNCTION; CHONDROCYTES; APOPTOSIS; SIRT1; PGC-1-ALPHA; BIOGENESIS; NETWORK; 1-ALPHA; KINASE;
D O I：
10.1159/000490418
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Background/Aims: Osteoarthritis is the most common degenerative joint disease and causes major pain and disability in adults. It has been reported that mitochondrial dysfunction in chondrocytes was associated with osteoarthritis. Puerarin has multiple effects including restoring mitochondrial function. In this study, the potential effects of puerarin on osteoarthritis and osteoarthritis associated mitochondrial dysfunctions were evaluated. Methods: Osteoarthritis rats were treated with puerarin and the severity of osteoarthritis and cartilage damages was evaluated. The mitochondrial biogenesis and functions were analyzed by measuring re- lated proteins expression, mitochondrial DNA content, ATP production, and oxygen consumption. The dependence of AMP-activated protein kinase (AMPK) pathway on puerarin-regulated mitochondrial function was analyzed by applying AMPK inhibitor Compound C. Results: Puerarin treatment alleviated mechanical hyperalgesia and cartilage damage in osteoarthritis rats. Puerarin increased mitochondrial biogenesis and attenuated mitochondrial dysfunctions in osteoarthritis rats. AMPK inhibitor Compound C abolished puerarin's effects. Conclusion: Puerarin attenuates osteoarthritis by upregulating the AMPK/proliferator-activated receptor-gamma coactivator signaling pathway in osteoarthritis rats. (C) 2018 S. Karger AG, Basel
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收藏
页码：117 / 125
页数：9
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