Characterization of quantum dots in cancer cytosol using ICP-MS-based combined techniques

被引：6

作者：

Kruszewska, Joanna ^{[1
]}

Matczuk, Magdalena ^{[1
]}

Skorupska, Sandra ^{[2
]}

Grabowska-Jadach, Ilona ^{[2
]}

Hernandez, Emma Perez ^{[1
]}

Timerbaev, Andrei ^{[3
]}

Jarosz, Maciej ^{[1
]}

机构：

[1] Warsaw Univ Technol, Fac Chem, Chair Analyt Chem, Noakowskiego St 3, PL-00664 Warsaw, Poland

[2] Warsaw Univ Technol, Fac Chem, Chair Med Biotechnol, Noakowskiego St 3, PL-00664 Warsaw, Poland

[3] Vernadsky Inst Geochem & Analyt Chem, Kosygin St 19, Moscow 119991, Russia

来源：

ANALYTICAL BIOCHEMISTRY | 2019年 / 584卷

关键词：

Capillary electrophoresis; High-performance liquid chromatography; Mass spectrometry; Quantum dots; Cancer cell; SIZE-EXCLUSION CHROMATOGRAPHY; ELEMENT SPECIATION ANALYSIS; CAPILLARY-ELECTROPHORESIS; SIGNAL AMPLIFICATION; GOLD NANOPARTICLES; PROTEIN CORONA; CE; SEPARATION; EFFICACY;

D O I：

10.1016/j.ab.2019.113387

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Knowledge of the intracellular behavior of quantum dots (QDs), which encompasses the antiproliferative effect on living cells, is still limited. For this reason, the transformations of CdSeS/ZnS-based QDs in cancer cytosol were examined using capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) hyphenated with inductively coupled plasma MS (ICP-MS). CE-ICP-MS method revealed the dose- and time-dependent speciation changes of QDs in the cytosol, while HPLC-ICP-MS (in the size-exclusion chromatography mode) allowed further characterization of the resulting Cd species. In such an appraisal, the decent CE advantage of high resolution is well complemented by higher sensitivity of HPLC (LOD 4.0 x 10(-10) and 5.4 x 10(-12) mol/L Cd, respectively). Additionally, the influence of serum protein corona on the surface of QDs on their uptake by Hep G2 cancer cells was investigated by direct ICP-MS analysis that revealed that the conjugated proteins greatly reduce the particle internalization.

引用

页数：5

共 28 条

[1] Capillary electrophoresis and nanomaterials - Part I: Capillary electrophoresis of nanomaterials [J].

Adam, Vojtech ;

Vaculovicova, Marketa .

ELECTROPHORESIS, 2017, 38 (19) :2389-2404

[2] Characterization of interactions of metal-containing nanoparticles with biomolecules by CE: An update (2012-2016) [J].

Aleksenko, Svetlana S. ;

Matczuk, Magdalena ;

Timerbaev, Andrei R. .

ELECTROPHORESIS, 2017, 38 (13-14) :1661-1668

[3] Interactions of tumour-targeting nanoparticles with proteins: potential of using capillary electrophoresis as a direct probe [J].

Aleksenko, Svetlana S. ;

Shmykov, Alexei Y. ;

Oszwaldowski, Slawomir ;

Timerbaev, Andrei R. .

METALLOMICS, 2012, 4 (11) :1141-1148

[4] The role of surface chemistry in serum protein corona-mediated cellular delivery and gene silencing with lipid nanoparticles [J].

Chen, Dongyu ;

Ganesh, Shanthi ;

Wang, Weimin ;

Amiji, Mansoor .

NANOSCALE, 2019, 11 (18) :8760-8775

[5] Quantum dots (QDs) restrain human cervical carcinoma HeLa cell proliferation through inhibition of the ROCK-c-Myc signaling [J].

Chen, Liqun ;

Qu, Guangbo ;

Zhang, Changwen ;

Zhang, Shuping ;

He, Jiuyang ;

Sang, Nan ;

Liu, Sijin .

INTEGRATIVE BIOLOGY, 2013, 5 (03) :590-596

[6] The therapeutic efficacy of CdTe and CdSe quantum dots for photothermal cancer therapy [J].

Chu, Maoquan ;

Pan, Xiaojing ;

Zhang, Ding ;

Wu, Qiang ;

Peng, Jinliang ;

Hai, Wangxi .

BIOMATERIALS, 2012, 33 (29) :7071-7083

[7] Capillary electrophoresis: the state-of-the-art in metal speciation studies [J].

Dabek-Zlotorzynska, E ;

Lai, EPC ;

Timerbaev, AR .

ANALYTICA CHIMICA ACTA, 1998, 359 (1-2) :1-26

[8] An improved protocol for ICP-MS-based assessment of the cellular uptake of metal-based nanoparticles [J].

Kruszewska, Joanna ;

Kur, Agnieszka ;

Kulpinska, Dominika ;

Grabowska-Jadach, Ilona ;

Matczuk, Magdalena ;

Keppler, Bernhard K. ;

Timerbaev, Andrei R. ;

Jarosz, Maciej .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2019, 174 :300-304

[9] Cellular processing of gold nanoparticles: CE-ICP-MS evidence for the speciation changes in human cytosol [J].

Legat, Joanna ;

Matczuk, Magdalena ;

Timerbaev, Andrei R. ;

Jarosz, Maciej .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2018, 410 (03) :1151-1156

[10] Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells [J].

Lesniak, Anna ;

Fenaroli, Federico ;

Monopoli, Marco R. ;

Aberg, Christoffer ;

Dawson, Kenneth A. ;

Salvati, Anna .

ACS NANO, 2012, 6 (07) :5845-5857

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