Characterization of quantum dots in cancer cytosol using ICP-MS-based combined techniques

被引:5
作者
Kruszewska, Joanna [1 ]
Matczuk, Magdalena [1 ]
Skorupska, Sandra [2 ]
Grabowska-Jadach, Ilona [2 ]
Hernandez, Emma Perez [1 ]
Timerbaev, Andrei [3 ]
Jarosz, Maciej [1 ]
机构
[1] Warsaw Univ Technol, Fac Chem, Chair Analyt Chem, Noakowskiego St 3, PL-00664 Warsaw, Poland
[2] Warsaw Univ Technol, Fac Chem, Chair Med Biotechnol, Noakowskiego St 3, PL-00664 Warsaw, Poland
[3] Vernadsky Inst Geochem & Analyt Chem, Kosygin St 19, Moscow 119991, Russia
关键词
Capillary electrophoresis; High-performance liquid chromatography; Mass spectrometry; Quantum dots; Cancer cell; SIZE-EXCLUSION CHROMATOGRAPHY; ELEMENT SPECIATION ANALYSIS; CAPILLARY-ELECTROPHORESIS; SIGNAL AMPLIFICATION; GOLD NANOPARTICLES; PROTEIN CORONA; CE; SEPARATION; EFFICACY;
D O I
10.1016/j.ab.2019.113387
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Knowledge of the intracellular behavior of quantum dots (QDs), which encompasses the antiproliferative effect on living cells, is still limited. For this reason, the transformations of CdSeS/ZnS-based QDs in cancer cytosol were examined using capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) hyphenated with inductively coupled plasma MS (ICP-MS). CE-ICP-MS method revealed the dose- and time-dependent speciation changes of QDs in the cytosol, while HPLC-ICP-MS (in the size-exclusion chromatography mode) allowed further characterization of the resulting Cd species. In such an appraisal, the decent CE advantage of high resolution is well complemented by higher sensitivity of HPLC (LOD 4.0 x 10(-10) and 5.4 x 10(-12) mol/L Cd, respectively). Additionally, the influence of serum protein corona on the surface of QDs on their uptake by Hep G2 cancer cells was investigated by direct ICP-MS analysis that revealed that the conjugated proteins greatly reduce the particle internalization.
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页数:5
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