Construction of glycosylated surfaces fox poly(propylene) beads with a photoinduced grafting/chemical reaction sequence

被引：10
作者：
Hu, Meng-Xin [1 ,2 ]
Wan, Ling-Shu [1 ,2 ]
Fu, Zhi-Sheng [1 ,2 ]
Fan, Zhi-Qiang [1 ,2 ]
Xu, Zhi-Kang [1 ,2 ]
机构：
[1] Zhejiang Univ, Minist Educ, Key Lab Macromol Synth & Functionalizat, Inst Polymer Sci, Hangzhou 310027, Peoples R China
[2] Zhejiang Univ, State Key Lab Chem Engn, Hangzhou 310027, Peoples R China
来源：
MACROMOLECULAR RAPID COMMUNICATIONS | 2007年 / 28卷 / 24期
关键词：
2-hydroxyethyl methacrylate; lectin; photografting; poly(propylene) beads; saccharides;
D O I：
10.1002/marc.200700487
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Surface modification of commercial PPBs with different saccharides is described. Surface-glycosylated PPBs were prepared through reaction between the hydroxyl groups of poly(HEMA) and acetylated saccharides such as a-glucose pentaacetate,,B-galactose pentaacetate, and lactose octaacetate. The modified PPBs were characterized by XPS and water contact angle measurement. It was found that the grafting degree of poly(HEMA) increases with UV irradiation time, monomer concentration, and water content in solvent. The binding degree of monosaccharides is higher than that of disaccharides. The glycosylated PPBs can selectively recognize lectins, indicating potential for protein isolation.
引用
收藏
页码：2325 / 2331
页数：7
相关论文
共 37 条
  • [1] Hydrophilic core-shell microspheres: A suitable support for controlled attachment of proteins and biomedical diagnostics
    Basinska, T
    [J]. MACROMOLECULAR BIOSCIENCE, 2005, 5 (12) : 1145 - 1168
  • [2] Overcoming degeneracy in carbohydrate recognition
    Basu, A
    Kahne, D
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (22) : 2504 - 2506
  • [3] Chemical surface modification of poly(ethylene terephthalate) fibers by aminolysis and grafting of carbohydrates
    Bech, Loic
    Meylheuc, Thierry
    Lepoittevin, Benedicte
    Roger, Philippe
    [J]. JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2007, 45 (11) : 2172 - 2183
  • [4] Chemical glycobiology
    Bertozzi, CR
    Kiessling, LL
    [J]. SCIENCE, 2001, 291 (5512) : 2357 - 2364
  • [5] Miniaturization of microwave-assisted carbohydrate functionalization to create oligosaccharide microarrays
    Brun, MA
    Disney, MD
    Seeberger, PH
    [J]. CHEMBIOCHEM, 2006, 7 (03) : 421 - 424
  • [6] Control of multivalent interactions by binding epitope density
    Cairo, CW
    Gestwicki, JE
    Kanai, M
    Kiessling, LL
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (08) : 1615 - 1619
  • [7] HIGH-SPEED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PEPTIDES AND PROTEINS
    CHEN, H
    HORVATH, C
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 705 (01) : 3 - 20
  • [8] Immobilisation on polystyrene of diazirine derivatives of mono- and disaccharides:: Biological activities of modified surfaces
    Chevolot, Y
    Martins, J
    Milosevic, N
    Léonard, D
    Zeng, S
    Malissard, M
    Berger, EG
    Maier, P
    Mathieu, HJ
    Crout, DHG
    Sigrist, H
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (11) : 2943 - 2953
  • [9] Merging organic and polymer chemistries to create glycomaterials for glycomics applications
    Coullerez, Geraldine
    Seeberger, Peter H.
    Textor, Marcus
    [J]. MACROMOLECULAR BIOSCIENCE, 2006, 6 (08) : 634 - 647
  • [10] Davis AP, 1999, ANGEW CHEM INT EDIT, V38, P2978
1234