Effects of pH and Ca2+ on heterodimer and heterotetramer formation by chromogranin A and chromogranin B

被引:40
作者
Yoo, SH [1 ]
Lewis, MS [1 ]
机构
[1] NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892
关键词
D O I
10.1074/jbc.271.29.17041
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The two major proteins of the secretory vesicles of neuroendocrine cells, chromogranin A (CGA) and chromogranin B (CGB), have been shown to undergo pH- and Ca2+-dependent conformational changes and aggregation and have been suggested to play essential roles during secretory vesicle biogenesis in the trans-Gels network, CGA has been shown to exist primarily in a tetrameric state at pH 5.5 and primarily in a dimeric state at pH 7.5, and CGB has been shown to exist in a monomeric state at both pH 5.5 and pH 7.5, Using purified CGA and CGB, it recently has been shown that CGA interacts with CGB at pH 5.5 (Yoo, S, H, (1996) J. Biol, Chem, 271, 1558-1565), In expanding this investigation, we have studied the temperature dependence of the pH-dependent interaction of CGA and CGB by analytical ultracentrifugation and found that two molecules of CGA bound to two molecules of CGB at pH 5.5 with Delta G(0) values of -43.6 kcal/mol in the absence of Ca2+ at 37 degrees C and -40.3 kcal/mol in the presence of 0.1 mM Ca2+, However, one molecule of CGA bound to one molecule of CGB at pH 7.5 with Delta G(0) values of -13.6 kcal/mol in the absence of Ca2+ at 37 degrees C, The magnitude of Delta G(0) values increased with increasing temperatures at both pH values, However, the values for enthalpy and entropy changes decreased with increasing temperatures in both pH levels, suggesting formation of more ordered structures, In the absence of Ca2+ at pH 5,5, the heterotetramerization reaction at 37 degrees C was entropically driven, whereas in the presence of Ca2+ (0.1 mM) the heterotetramerization was virtually an enthalpic reaction, On the other hand, the heterodimer formation in the absence of Ca2+ at pH 7.5 showed large negative enthalpy and entropy changes at 37 degrees C, indicating an enthalpic interaction compensated by entropic changes, In view of the interaction of tetrameric CGA with tetrameric inositol 1,4,5-trisphosphate (Ip(3)) receptor and the existence of heterotetrameric IP3 receptor in the cell, the heterotetramer formation by CGA and CGB not only raises the possibility of interaction between the heterotetrameric chromogranin and heterotetrameric IP3 receptor but also appears to reflect their important roles in the cell.
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页码:17041 / 17046
页数:6
相关论文
共 31 条
[1]   VESICLES AND CISTERNAE IN THE TRANS GOLGI-APPARATUS OF HUMAN-FIBROBLASTS ARE ACIDIC COMPARTMENTS [J].
ANDERSON, RGW ;
PATHAK, RK .
CELL, 1985, 40 (03) :635-643
[2]   PRIMARY STRUCTURE OF BOVINE CHROMOGRANIN-B DEDUCED FROM CDNA SEQUENCE [J].
BAUER, JW ;
FISCHERCOLBRIE, R .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1089 (01) :124-126
[3]   THE PRIMARY STRUCTURE OF BOVINE CHROMOGRANIN-A - A REPRESENTATIVE OF A CLASS OF ACIDIC SECRETORY PROTEINS COMMON TO A VARIETY OF PEPTIDERGIC CELLS [J].
BENEDUM, UM ;
BAEUERLE, PA ;
KONECKI, DS ;
FRANK, R ;
POWELL, J ;
MALLET, J ;
HUTTNER, WB .
EMBO JOURNAL, 1986, 5 (07) :1495-1502
[4]   ALL-OR-NOTHING CA2+ MOBILIZATION FROM THE INTRACELLULAR STORES OF SINGLE HISTAMINE-STIMULATED HELA-CELLS [J].
BOOTMAN, MD ;
BERRIDGE, MJ ;
TAYLOR, CW .
JOURNAL OF PHYSIOLOGY-LONDON, 1992, 450 :163-178
[5]   MILIEU-INDUCED, SELECTIVE AGGREGATION OF REGULATED SECRETORY PROTEINS IN THE TRANS-GOLGI NETWORK [J].
CHANAT, E ;
HUTTNER, WB .
JOURNAL OF CELL BIOLOGY, 1991, 115 (06) :1505-1519
[6]   INTERACTION OF CALCIUM WITH PORCINE ADRENAL CHROMOGRANIN-A (SECRETORY PROTEIN-I) AND CHROMOGRANIN-B (SECRETOGRANIN-I) [J].
GORR, SU ;
SHIOI, J ;
COHN, DV .
AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (02) :E247-E254
[7]   THE INSULIN SECRETORY GRANULE [J].
HUTTON, JC .
DIABETOLOGIA, 1989, 32 (05) :271-281
[8]   BOVINE CHROMOGRANIN-A SEQUENCE AND DISTRIBUTION OF ITS MESSENGER-RNA IN ENDOCRINE TISSUES [J].
IACANGELO, A ;
AFFOLTER, HU ;
EIDEN, LE ;
HERBERT, E ;
GRIMES, M .
NATURE, 1986, 323 (6083) :82-86
[9]   HETEROLIGOMERS OF TYPE-I AND TYPE-III INOSITOL TRISPHOSPHATE RECEPTORS IN WB RAT-LIVER EPITHELIAL-CELLS [J].
JOSEPH, SK ;
LIN, C ;
PIERSON, S ;
THOMAS, AP ;
MARANTO, AR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (40) :23310-23316
[10]  
LEWIS MS, 1994, MODERN ANAL ULTRACEN, P94