Whey protein supplementation increases methionine intake but not homocysteine plasma concentration in rats

被引:9
|
作者
Deminice, Rafael [1 ,2 ]
Comparotto, Hugo [1 ]
Jordao, Alceu Afonso [1 ]
机构
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Nutr & Metab, BR-14049 Ribeirao Preto, SP, Brazil
[2] Univ Estadual Londrina, Fac Phys Educ & Sport, Dept Phys Educ, Londrina, Parana, Brazil
关键词
protein supplements; hyperhomocysteinemia; oxidative stress; liver; glutathione; VASCULAR ENDOTHELIAL DYSFUNCTION; CREATINE SUPPLEMENTATION; OXIDATIVE STRESS; HYPERHOMOCYSTEINEMIA; REQUIREMENTS; GLUTATHIONE; METABOLISM; DIET;
D O I
10.1139/apnm-2014-0175
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The purpose of this study was to examine the effects of whey protein supplementation on homocysteine (Hcy) metabolism and liver oxidative stress in rats. Twenty-four rats were divided into 3 groups (n = 8) to receive one of the following diets for 4 weeks: control diet (C), whey protein-composed diet (WP), and whey protein-supplemented diet (WPS). The C and WP diets consisted of AIN-93 with 20% casein and 20% whey protein as protein source, respectively. WPS was AIN-93 (20% casein) supplemented by the addition of 20% (w/w) whey protein. Four weeks of ingesting a WPS diet resulted in a significantly higher (P < 0.05) total protein and methionine intakes. Although a significant increase (P < 0.05) in the hepatic S-adenosylmethionine and S-adenosylhomocysteine levels occurred in WPS group compared with C and WP, no significant change was observed in plasma Hcy concentration between groups. Furthermore, the levels of lipid hydroperoxides and advanced oxidation protein products, known liver oxidative stress markers, were increased in the WPS group compared with the C group. In addition, no change in glutathione liver concentration was observed in any of the groups studied. In conclusion, whey protein supplementation increases methionine intake substantially; however, it does not change plasma Hcy concentrations. On the other hand, increased hepatic oxidative stress markers were observed in whey protein supplemented rats were probably due to high protein intake.
引用
收藏
页码:46 / 50
页数:5
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