AFM analysis of the formation of DNA aggregates on polymeric biochips

被引：1
|
作者：
Sawant, PD [1 ]
Watson, G [1 ]
Myhra, S [1 ]
Nicolau, D [1 ]
机构：
[1] Swinburne Univ Technol, Ind Res Inst, Hawthorn, Vic 3122, Australia
来源：
BIOMEDICAL APPLICATIONS OF MICRO- AND NANOENGINEERING II | 2005年 / 5651卷
关键词：
DNA immobilization; DNA hybridization; atomic force microscopy; fractal dimensions; DNA microarrays;
D O I：
10.1117/12.584142
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The demand for polymer-based DNA microarrays will increase because of their cost-effectiveness, biocompatibility and easy processing. However not all polymers are ideal substrates because of different chemical interactions of polymeric substrates with the DNA molecules. Results from AFM analysis of DNA immobilised on polymeric surfaces are evaluated using fractality, Gaussian distribution and lateral force imaging. It has been found that the nanosize defects in the substrate, such as poly-1-lysine, plays an important role in the growth of DNA aggregates in a vertical direction, whereas the covalent binding of DNA molecules on NHS-functionalised cyclo-olefin copolymer leads to the lateral growth of DNA aggregates.
引用
收藏
页码：153 / 159
页数：7
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