Minichromosomes derived from the B chromosome of maize

被引：34

作者：

Kato, A ^{[1
]}

Zheng, YZ ^{[1
]}

Auger, DL ^{[1
]}

Phelps-Durr, T ^{[1
]}

Bauer, MJ ^{[1
]}

Lamb, JC ^{[1
]}

Birchler, JA ^{[1
]}

机构：

[1] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA

来源：

CYTOGENETIC AND GENOME RESEARCH | 2005年 / 109卷 / 1-3期

关键词：

D O I：

10.1159/000082395

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fourteen minichromosomes derived from the B chromosome of maize are described. The centromeric region of the B chromosome contains a specific repetitive DNA element called the B repeat. This sequence was used to determine the transmission frequency of the different types of minichromosomes over several generations via Southern blot analysis at each generation. In general, the minichromosomes have transmission rates below the theoretical 50% frequency of a univalent chromosome. The gross structure of each minichromosome was determined using fluorescence in situ hybridization ( FISH) on root tip chromosome spreads. The presence of the B centromeric repeat and of the adjacent heterochromatic knob sequences was determined for each minichromosome. In two cases, the amount of the centromeric knob repeat is increased relative to the progenitor chromosome. Other isolates have reduced or undetectable levels of the knob sequence. Potential uses of the minichromosomes are discussed. Copyright (C) 2005 S. Karger AG, Basel.

引用

页码：156 / 165

页数：10

共 36 条

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