Enhanced electrochemical biosensing of alpha-fetoprotein based on three-dimensional macroporous conducting polymer polyaniline

被引：53
作者：
Liu, Shuo [1 ]
Ma, Yihui [1 ]
Cui, Min [2 ]
Luo, Xiliang [1 ]
机构：
[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China
[2] Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 255卷
基金：
中国国家自然科学基金;
关键词：
Conducting polymer; Electropolymerization; Alpha fetoprotein; Immunosensor; Macroporous materials; Polyaniline; SODIUM DODECYL-SULFATE; CARBON NANOTUBES; SIGNAL AMPLIFICATION; IMMUNOSENSOR; NANOPARTICLES; IMMUNOASSAY; PROTEIN; FABRICATION;
D O I：
10.1016/j.snb.2017.09.062
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive and selective electrochemical sensor for the determination of alpha-fetoprotein (AFP) was developed based on the three-dimensional (3D) macroporous polyaniline (PANI) doped with poly( sodium 4-styrene sulfonate) (PSS) by using a hard-template method. The 3D macroporous PANI possessed large surface area, high conductivity and many functional groups, and it provided an excellent substrate for the immobilization of alpha-fetoprotein (AFP) antibodies to construct an electrochemical biosensor. Based on the measurement of the inherent redox signal change of the porous PANI, the prepared AFP immunosensor was reagentless and it exhibited satisfying sensing performances toward its target AFP. Under optimum conditions, the porous PANI-based immunosensor achieved a wide linear range for AFP from 0.01 to 1000 pg mL(-1), with a detection limit of 3.7 fg mL(-1). Significantly, the response sensitivity of this sensor was about 2 times higher than that of the planar PANI-modified electrode, manifesting a great enhancement effect associated with the 3D macroporous structure. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：2568 / 2574
页数：7
相关论文
共 62 条
[1]   Gold nanoparticles based sandwich electrochemical immunosensor [J].
Ahirwal, Gautham Kumar ;
Mitra, Chanchal K. .
BIOSENSORS & BIOELECTRONICS, 2010, 25 (09) :2016-2020
[2]   Enhanced sensitivity of a gas sensor incorporating single-walled carbon nanotube-polypyrrole nanocomposites [J].
An, KH ;
Jeong, SY ;
Hwang, HR ;
Lee, YH .
ADVANCED MATERIALS, 2004, 16 (12) :1005-+
[3]  
[Anonymous], 2008, CONDUCTING POLYM MIC
[4]   Gas sensors based on conducting polymers [J].
Bai, Hua ;
Shi, Gaoquan .
SENSORS, 2007, 7 (03) :267-307
[5]   Conducting-polymer microcontainers: Controlled syntheses and potential applications [J].
Bajpai, V ;
He, PG ;
Dai, LM .
ADVANCED FUNCTIONAL MATERIALS, 2004, 14 (02) :145-151
[6]   Single Conducting Polymer Nanowire Chemiresistive Label-Free Immunosensor for Cancer Biomarker [J].
Bangar, Mangesh A. ;
Shirale, Dhammanand J. ;
Chen, Wilfred ;
Myung, Nosang V. ;
Mulchandani, Ashok .
ANALYTICAL CHEMISTRY, 2009, 81 (06) :2168-2175
[7]   Electrochemical syntheses of highly ordered macroporous conducting polymers grown around self-assembled colloidal templates [J].
Bartlett, PN ;
Birkin, PR ;
Ghanem, MA ;
Toh, CS .
JOURNAL OF MATERIALS CHEMISTRY, 2001, 11 (03) :849-853
[8]   Multilayers enzyme-coated carbon nanotubes as biolabel for ultrasensitive chemiluminescence immunoassay of cancer biomarker [J].
Bi, Sai ;
Zhou, Hong ;
Zhang, Shusheng .
BIOSENSORS & BIOELECTRONICS, 2009, 24 (10) :2961-2966
[9]   Electrochemical polymerization of aniline monomers infiltrated into well-ordered truncated eggshell structures of polyelectrolyte multilayers [J].
Briseno, AL ;
Han, SB ;
Rauda, IE ;
Zhou, FM ;
Toh, CS ;
Nemanick, EJ ;
Lewis, NS .
LANGMUIR, 2004, 20 (01) :219-226
[10]   An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood [J].
Bryan, Thomas ;
Luo, Xiliang ;
Bueno, Paulo R. ;
Davis, Jason J. .
BIOSENSORS & BIOELECTRONICS, 2013, 39 (01) :94-98
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