Comprehensive analysis of transcriptomics and metabolomics to understand the flesh quality regulation of crucian carp (Carassius auratus) treated with short term micro-flowing water system

被引：36

作者：

Du, Hongying ^{[1
,2
]}

Xiong, Shanbai ^{[1
,2
]}

Lv, Hao ^{[1
]}

Zhao, Siming ^{[1
,2
]}

Manyande, Anne ^{[3
]}

机构：

[1] Huazhong Agr Univ, Coll Food Sci & Technol, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan, Hubei, Peoples R China

[2] Natl R&D Branch Ctr Convent Freshwater Fish Proc, Wuhan 430070, Hubei, Peoples R China

[3] Univ West London, Sch Human & Social Sci, Brentford TW8 9GA, Middx, England

来源：

FOOD RESEARCH INTERNATIONAL | 2021年 / 147卷

基金：

中国国家自然科学基金;

关键词：

Freshwater fish; Mass spectrometry (MS); Genes; Metabolites; Principal component analysis (PCA); Orthogonal partial least squares discriminant analysis (OPLS-DA); FILLET QUALITY; WILD; MECHANISMS; EXPOSURE; PERFORMANCE; DEPURATION; METABOLISM; PROFILES; IMPACT;

D O I：

10.1016/j.foodres.2021.110519

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The short term micro-flowing purification system (STMFPS) has been shown to improve the flesh quality of freshwater fish. However, few studies have focused on the involved underlying mechanisms. This study explored the effect of STMFPS on the flesh quality of market-size freshwater fish based on the combination of metabolomics and transcriptomics methods. The UPLC-QTOF/MS based metabolomics method was utilized to screen metabolites and predict the possible major metabolic pathways during different STMFPS treatment periods (0 d, 1 d, 5 d and 9 d). Furthermore, the transcriptomic data demonstrated that the differentially expressed genes detected in crucian carp muscle were 2915, 7852 and 7183 after 1 d, 5 d and 9 d STMFPS treatment. Results showed that the TCA cycle, ornithine cycle, purine metabolism and amino acid catabolism play important roles in improving the flesh quality of crucian carp. This study may help to understand the mechanism of improving the flesh quality of aquatic products using STMFPS.

引用

页数：13

共 44 条

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