Identification of an mRNP Complex Regulating Tumorigenesis at the Translational Elongation Step

被引：160

作者：

Hussey, George S. ^{[1
,4
]}

Chaudhury, Arindam ^{[1
,4
]}

Dawson, Andrea E. ^{[2
]}

Lindner, Daniel J. ^{[3
]}

Knudsen, Charlotte R. ^{[5
]}

Wilce, Matthew C. J. ^{[6
]}

Merrick, William C. ^{[7
]}

Howe, Philip H. ^{[1
]}

机构：

[1] Cleveland Clin, Dept Canc Biol, Lerner Res Inst, Cleveland, OH 44195 USA

[2] Cleveland Clin, Dept Anat Pathol, Cleveland, OH 44195 USA

[3] Cleveland Clin, Dept Translat Hematol & Oncol Res, Cleveland, OH 44195 USA

[4] Cleveland State Univ, Dept Biol Geol & Environm Sci, Cleveland, OH 44115 USA

[5] Univ Aarhus, Dept Mol Biol, DK-8000 Aarhus C, Denmark

[6] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3168, Australia

[7] Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA

来源：

MOLECULAR CELL | 2011年 / 41卷 / 04期

关键词：

EPITHELIAL-MESENCHYMAL TRANSITION; MESSENGER-RNA; TGF-BETA; PROTEIN-SYNTHESIS; CELL-MIGRATION; FACTOR; 1-ALPHA; FACTOR-TU; METASTASIS; CANCER; INHIBITION;

D O I：

10.1016/j.molcel.2011.02.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcript-selective translational regulation of epithelial-mesenchymal transition (EMT) by transforming growth factor-beta (TGF-beta) is directed by the hnRNP E1-containing TGF-beta-activated-translational (BAT) mRNP complex. Herein, eukaryotic elongation factor-1 A1 (eEF1A1) is identified as an integral component of the BAT complex. Translational silencing of Dab2 and ILEI, two EMT transcripts, is mediated by the binding of hnRNP E1 and eEF1A1 to their 3'UTR BAT element, whereby hnRNP E1 stalls translational elongation by inhibiting the release of eEF1A1 from the ribosomal A site. TGF-beta-mediated hnRNP E1 phosphorylation, through Akt2, disrupts the BAT complex, thereby restoring translation of target EMT transcripts. Attenuation of hnRNP expression in two noninvasive breast epithelial cells (NMuMG and MCF-7) not only induced EMT but also enabled cells to form metastatic lesions in vivo. Thus, translational regulation by TGF-beta at the elongation stage represents a critical checkpoint coordinating the expression of EMT transcripts required during development and in tumorigenesis and metastatic progression.

引用

页码：419 / 431

页数：13

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