Simple surface functionalization of magnetic nanoparticles with methotrexate-conjugated bovine serum albumin as a biocompatible drug delivery vehicle

被引：10
作者：
Azizi, Sedigheh [1 ]
Nosrati, Hamed [1 ,2 ]
Danafar, Hossein [1 ,2 ]
机构：
[1] Zanjan Univ Med Sci, Zanjan Pharmaceut Nanotechnol Res Ctr, Zanjan, Iran
[2] Zanjan Univ Med Sci, Sch Pharm, Dept Pharmaceut Biomat, Zanjan, Iran
来源：
APPLIED ORGANOMETALLIC CHEMISTRY | 2020年 / 34卷 / 04期
关键词：
bovine serum albumin (BSA); enzyme-dependent release; magnetic nanoparticles; methotrexate (MTX); protein-based vehicle; IRON-OXIDE NANOPARTICLES; SUPERPARAMAGNETIC NANOPARTICLES; CANCER; MICELLES; RELEASE; COMBINATION; THERAPY; ENHANCE;
D O I：
10.1002/aoc.5479
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Magnetic nanoparticles (MNPs) functionalized with methotrexate (MTX)-conjugated bovine serum albumin (BSA) as a biocompatible drug delivery vehicle were synthesized using a facile method. Characterization of the functionalized MNPs (Fe3O4@BSA-MTX NPs) was performed using various techniques including UV-visible spectroscopy, dynamic light scattering, vibrating sample magnetometry and X-ray diffraction. The particle size and zeta potential of Fe3O4@BSA-MTX NPs were 105.7 +/- 3.81 nm (mean +/- SD) and -18.2 mV, respectively. MTX release from Fe3O4@BSA-MTX NPs showed an enzyme-dependent release pattern. Hemo-biocompatibility of Fe3O4@BSA-MTX NPs was confirmed using hemolysis test. In addition, the cytotoxicity of functionalized MNPs and free MTX against MCF-7 cell line was investigated using MTT assay. The results of experiments revealed that the Fe3O4@BSA-MTX NPs as a biocompatible carrier could improve the therapeutic effect of MTX.
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页数：9
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