The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles

被引：2

作者：

Sorokina, O. N. ^{[1
]}

Bychkova, A. V. ^{[1
]}

Shapiro, A. B. ^{[1
]}

Tikhonov, A. P. ^{[2
]}

Kovarskii, A. L. ^{[1
]}

机构：

[1] Russian Acad Sci, Emanuel Inst Biochem Phys, Moscow 119334, Russia

[2] Mendeleev Univ Chem Technol, Moscow 125047, Russia

来源：

RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY B | 2010年 / 4卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Magnetite; Electron Paramagnetic Resonance; Electron Paramagnetic Resonance Spectrum; Spin Label; Electron Paramagnetic Resonance Line;

D O I：

10.1134/S199079311003022X

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

A procedure for using spin-label EPR spectroscopy in studies of the adsorption of macromolecular systems on magnetic nanoparticles and quantitative characterization of adsorption layers was developed. The procedure was based on measurements of the intensity of EPR signals of stable nitroxide radical labels covalently bound with macromolecules. The adsorption of spin-labeled macromolecules on the surface of nanoparticles caused broadening of EPR lines as a result of the dipole interaction of unpaired radical electrons with the magnetic moment of particles, which decreased signal intensity. Multilayer adsorption in aqueous media of biological fibrinogen molecules and synthetic polymer (polyethyleneimine) on magnetite particles with a mean diameter of 17 nm was studied. The method was shown to be applicable to the estimation of the amount of adsorbed macromolecules in multilayer coatings up to similar to 40 nm thick.

引用

页码：510 / 514

页数：5

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