Caspase-independent autophagic cytotoxicity in etoposide-treated CaSki cervical carcinoma cells

被引:31
作者
Lee, Seung-Baek
Tong, Seo-Yun
Kim, Jung-Jin
Um, Soo-Jong
Park, Jong-Sup [1 ]
机构
[1] Catholic Univ, Coll Med, Grad Sch Med, Dept Med Biosci, Seoul, South Korea
[2] Korea Univ, Korea Univ Coll Med, Grad Sch Med, Seoul 136701, South Korea
[3] Kyung Hee Univ, EW Neo Med Ctr, Dept Obstet & Gynecol, Seoul, South Korea
[4] Sejong Univ, Dept Biosci & Biotechnol, Seoul, South Korea
[5] Catholic Univ, Coll Med, Dept Obstet & Gynecol, Div Gynecol Oncol, Seoul, South Korea
关键词
MALIGNANT GLIOMA-CELLS; TOPOISOMERASE-II; CANCER-CELLS; DEATH; MECHANISM; RAT; INHIBITION; RELIGATION; RADIATION; PROTEINS;
D O I
10.1089/dna.2007.0577
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We studied the in vitro mechanism of etoposide- induced cell death in cervical cancer cells. Etoposide is cytotoxic to these cells, causing cell death by both apoptosis and autophagy, which has recently been described as a possible mechanism for nonapoptotic cell death. Electron microscopy revealed that autophagosomes/autolysosomes exhibited an autophagic appearance in the presence of etoposide. When autophagy was blocked by inhibitors of autophagy, including 3-methyladenine, both the expression of beclin 1 protein and the antitumor effect of etoposide were suppressed. Benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone, a pan-caspase inhibitor, reduced etoposide-induced cytotoxicity in CaSki cells. Hence, autophagy and apoptosis likely occur concurrently in etoposide-treated cervical cancer cells.
引用
收藏
页码:713 / U1
页数:9
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