Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

被引：122

作者：

Ding, Yidan ^{[1
]}

Li, Jiao ^{[2
]}

Enterina, Jhon Ralph ^{[1
]}

Shen, Yi ^{[1
]}

Zhang, Issan ^{[3
]}

Tewson, Paul H. ^{[4
]}

Mo, Gary C. H. ^{[5
]}

Zhang, Jin ^{[5
]}

Quinn, Anne Marie ^{[4
]}

Hughes, Thomas E. ^{[4
,6
]}

Maysinger, Dusica ^{[3
]}

Alford, Spencer C. ^{[1
,7
]}

Zhang, Yan ^{[2
]}

Campbell, Robert E. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB, Canada

[2] Peking Univ, McGovern Inst Brain Res, State Key Lab Biomembrane & Membrane Biotechnol, Coll Life Sci,Int Data Grp, Beijing 100871, Peoples R China

[3] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ, Canada

[4] Montana Mol, Bozeman, MT USA

[5] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[6] Montana State Univ, Dept Cell Biol & Neurosci, Bozeman, MT 59717 USA

[7] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2015年 / 12卷 / 03期

基金：

加拿大健康研究院; 美国国家科学基金会; 美国国家卫生研究院; 北京市自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

ENERGY-TRANSFER; CELL-DEATH; GREEN; CA2+; SIGNAL;

D O I：

10.1038/nmeth.3261

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca2+ concentration dynamics and other second-messenger signaling activities.

引用

页码：195 / +

页数：7

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