Hydrogen peroxide induces protection against lethal effects of cumene hydroperoxide in Escherichia coli cells:: an Ahp dependent and OxyR independent system?

被引：18
作者：
Asad, NR [1 ]
Asad, LMBO
Silva, AB
Felzenszwalb, I
Leitao, C
机构：
[1] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Biofis & Biometria, BR-20551030 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Radiobiol Mol, BR-21949900 Rio De Janeiro, Brazil
来源：
MUTATION RESEARCH-DNA REPAIR | 1998年 / 407卷 / 03期
关键词：
hydrogen peroxide; alkyl hydroperoxide reductase; Escherichia coli; cumene hydroperoxide;
D O I：
10.1016/S0921-8777(98)00010-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Pretreatment with 2.5 mM H2O2 protects bacterial cells against cumene hydroperoxide killing. This response is independent of the OxyR system, but possibly involves the participation of Ahp protein, since ahp mutants are not protected. Treatment of bacterial cells with high H2O2 concentrations caused an alteration on the electrophoretic profile of the smaller subunit (22-kDa) of Ahp. This alteration does not require novel gene products and is not dependent on the OxyR protein. In this way, we propose that the modification of the 22-kDa subunit of Ahp by high H2O2 concentration may be responsible for the protection against the lethal effects of cumene hydroperoxide. (C) 1998 Elsevier Science B.V. All rights reserved.
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收藏
页码：253 / 259
页数：7
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