DNA hybridization assays using metal-enhanced fluorescence

被引：102

作者：

Malicka, J ^{[1
]}

Gryczynski, I ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] Univ Maryland, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 01期

关键词：

DNA hybridization; metal-enhanced fluorescence; silver nanoparticles;

D O I：

10.1016/S0006-291X(03)00935-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity during hybridization. Proximity to silver particles resulted in a decreased fluorescence lifetime. This effect is thought to be the result of enhanced fluorescence from fluorescein near metallic silver particles. Hybridization could thus be measured from the decay kinetics of the emission, which can be measured independently from the emission intensity. These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：213 / 218

页数：6

共 19 条

[1]

Amrbose W. P., 1999, CHEM REV, V99, P2929

[2]

Barnes WL, 1998, J MOD OPTIC, V45, P661, DOI 10.1080/09500349808230614

[3] Exploring the new world of the genome with DNA microarrays [J].

Brown, PO ;

Botstein, D .

NATURE GENETICS, 1999, 21 (Suppl 1) :33-37

[4]

DIFILIPPANTONIO MJ, 2003, TOPICS FLUORESCENCE, V7, P291

[5] Quantitation of HCV RNA using real-time PCR and fluorimetry [J].

Komurian-Pradel, F ;

Paranhos-Baccalà, G ;

Sodoyer, M ;

Chevallier, P ;

Mandrand, B ;

Lotteau, V ;

André, P .

JOURNAL OF VIROLOGICAL METHODS, 2001, 95 (1-2) :111-119

[6] ENHANCED DYE FLUORESCENCE OVER SILVER ISLAND FILMS - ANALYSIS OF THE DISTANCE DEPENDENCE [J].

KUMMERLEN, J ;

LEITNER, A ;

BRUNNER, H ;

AUSSENEGG, FR ;

WOKAUN, A .

MOLECULAR PHYSICS, 1993, 80 (05) :1031-1046

[7] A 10-GHZ FREQUENCY-DOMAIN FLUOROMETER [J].

LACZKO, G ;

GRYCZYNSKI, I ;

GRYCZYNSKI, Z ;

WICZK, W ;

MALAK, H ;

LAKOWICZ, JR .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1990, 61 (09) :2331-2337

[8]

Lakowicz J.R., 2004, PRINCIPLES FLUORESCE, VSecond

[9] Radiative decay engineering: Biophysical and biomedical applications [J].

Lakowicz, JR .

ANALYTICAL BIOCHEMISTRY, 2001, 298 (01) :1-24

[10] Radiative decay engineering 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer [J].

Lakowicz, JR ;

Shen, YB ;

D'Auria, S ;

Malicka, J ;

Fang, JY ;

Gryczynski, Z ;

Gryczynski, I .

ANALYTICAL BIOCHEMISTRY, 2002, 301 (02) :261-277

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