Genetic Variability of the AcrAB-TolC Multidrug Efflux Pump Underlies SkQ1 Resistance in Gram-Negative Bacteria

被引：9
作者：
Nazarov, P. A. [1 ,2 ]
Kotova, E. A. [1 ]
Skulachev, V. P. [1 ,3 ]
Antonenko, Y. N. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow 119991, Russia
[2] Mitotech LLC, Moscow 119991, Russia
[3] Lomonosov Moscow State Univ, Inst Mitoengn, Moscow 119991, Russia
来源：
ACTA NATURAE | 2019年 / 11卷 / 04期
基金：
俄罗斯科学基金会;
关键词：
SkQ1; AcrZ; AcrAB-TolC efflux pump; multidrug resistance; MITOCHONDRIA;
D O I：
10.32607/20758251-2019-11-4-93-98
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SkQ1, a novel antibiotic targeting bacterial bioenergetics, is highly effective against both gram-positive and gram-negative bacteria. However, some gram-negative bacteria, such as Escherichia coli and Klebsiella pneumoniae, are highly resistant to it. In different gram-negative bacteria, this resistance is associated with the identity of their AcrB transporter protein sequence with the sequence of the AcrB protein from E. coli. SkQ1 is expelled from E. coli cells by the AcrAB-TolC multidrug efflux pump. In this study, we demonstrate that SkQ1 resistance in E. coli, in contrast to chloramphenicol resistance, does not depend on the presence of the multidrug efflux pump accessory protein AcrZ.
引用
收藏
页码：93 / 98
页数：6
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