Identification of meat species in different types of meat products by PCR

被引:0
作者
Keyvan, Erhan [1 ]
Iplikcioglu Cil, Guzin [2 ]
Cinar Kul, Bengi
Bilgen, Nuket
Sireli, Ufuk Tansel [2 ]
机构
[1] Mehmet Akif Ersoy Univ, Fac Vet Med, Dept Food Hyg & Technol, Burdur, Turkey
[2] Ankara Univ, Fac Vet Med, Dept Food Hyg & Technol, Ankara, Turkey
来源
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI | 2017年 / 64卷 / 04期
关键词
Adulteration; meat species identification; mislabeling; PCR; LINKED-IMMUNOSORBENT-ASSAY; POLYMERASE-CHAIN-REACTION; RAW; AUTHENTICATION; SUBSTITUTION; FEEDSTUFFS; FOOD; DNA;
D O I
10.1501/Vetfak_0000002808
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Species identification in food of animal origin is an important subject for food control. Adulteration in foods is relevant for economical, religous, legislation or public health concerning reasons. The aim of this study was to determine the adulteration in different meat products such as sucuk (n=37), sausage (n=33) and salami (n=32). A total of 102 different meat products obtained from various markets in Ankara were analyzed for species identification by PCR. According to the analyzes, five (13.5%) poultry, and one (2.7%) poultry and equine meats together were found in the sucuk samples that are not declared in their labels. Also, seven (21.8%) and two (6.1%) poultry meat were detected in 32 salami and 33 sausage samples, respectively. These results indicate that 15 (14.7%) of the total samples were found to contain undeclared species. As a result, there were meat products which were not in compliance with their labels in various markets, presenting a potential public health risk and economical losses of consumers.
引用
收藏
页码:261 / 266
页数:6
相关论文
共 26 条
[1]  
[Anonymous], 2014, LIVESTOCK PRODUCTION
[2]  
[Anonymous], 2012, TURKISH FOOD CODEX C
[3]   Detection of species in meat and meat products using enzyme-linked immunosorbent assay [J].
Ayaz, Y ;
Ayaz, ND ;
Erol, I .
JOURNAL OF MUSCLE FOODS, 2006, 17 (02) :214-220
[4]   Authentication of meat and meat products [J].
Ballin, N. Z. .
MEAT SCIENCE, 2010, 86 (03) :577-587
[5]   Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures [J].
Camma, Cesare ;
Di Domenico, Marco ;
Monaco, Federica .
FOOD CONTROL, 2012, 23 (02) :400-404
[6]   A high incidence of species substitution and mislabelling detected in meat products sold in South Africa [J].
Cawthorn, Donna-Maree ;
Steinman, Harris A. ;
Hoffman, Louwrens C. .
FOOD CONTROL, 2013, 32 (02) :440-449
[7]   Identification of the goose species (Anser anser) in Italian "Mortara" salami by DNA sequencing and a Polymerase Chain Reaction with an original primer pair [J].
Colombo, F ;
Marchisio, E ;
Pizzini, A ;
Cantoni, C .
MEAT SCIENCE, 2002, 61 (03) :291-294
[8]   A multiplex PCR assay for the identification of animal species in feedstuffs [J].
Dalmasso, A ;
Fontanella, E ;
Piatti, P ;
Civera, T ;
Rosati, S ;
Bottero, MT .
MOLECULAR AND CELLULAR PROBES, 2004, 18 (02) :81-87
[9]   Detection of meat species using TaqMan real-time PCR assays [J].
Dooley, JJ ;
Paine, KE ;
Garrett, SD ;
Brown, HM .
MEAT SCIENCE, 2004, 68 (03) :431-438
[10]   Fraud identification in industrial meat products by multiplex PCR assay [J].
Ghovvati, S. ;
Nassiri, M. R. ;
Mirhoseini, S. Z. ;
Moussavi, A. Heravi ;
Javadmanesh, A. .
FOOD CONTROL, 2009, 20 (08) :696-699