Comparative proteomic profiling of mammalian cell lysates using phosphopeptide microarrays

被引：28

作者：

Gao, Liqian ^{[1
,2
]}

Uttamchandani, Mahesh ^{[1
,3
,4
]}

Yao, Shao Q. ^{[1
,2
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] ZNatl Univ Singapore, Off Life Sci, NUS MedChem Program, Singapore 117543, Singapore

[3] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[4] DSO Natl Labs, Def Med & Environm Res Inst, Singapore 117510, Singapore

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 16期

关键词：

GROWTH-FACTOR RECEPTOR; PEPTIDE MICROARRAY; THROUGHPUT; PHOSPHORYLATION; IDENTIFICATION; SPECIFICITY; PHOSPHATASE; GRB2;

D O I：

10.1039/c2cc17701c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A library of 176 human phosphotyrosine-containing peptides was used to establish cell lysate binding profiles in a two colour microarray format. The resulting hits led to the pull-down and identification of biomarkers associated with cancer states.

引用

页码：2240 / 2242

页数：3

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