Development of primary culture from heart tissue of Chitala chitala (Hamilton-Buchanan)

被引：0

作者：

Kapoor, Smita ^{[1
]}

Bhatt, Kamalendra J. P. ^{[1
]}

Lakra, W. S. ^{[1
]}

Rathore, G. ^{[1
]}

Goswami, M. ^{[1
]}

Swaminathan, T. Raja ^{[1
]}

机构：

[1] Natl Bur Fish Genet Resources, Lucknow 226002, Uttar Pradesh, India

来源：

INDIAN JOURNAL OF ANIMAL SCIENCES | 2012年 / 82卷 / 01期

关键词：

Chital; Heart; Primary culture; EPINEPHELUS-AWOARA TEMMINCK; INDIAN MAJOR CARP; CELL-LINES; GROUPER; IRIDOVIRUS; KIDNEY; LIVER; ESTABLISHMENT; SCHLEGEL; COIOIDES;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A method for developing cell cultures from Chitala chitala heart tissue is described. The tissues were minced and seeded in culture flasks and grown in Leibovitz L-15 medium supplemented with 20% foetal bovine serum. The radiation started after 4th day in heart. The medium was changed after every five days. The radiations were able to withstand a wide range of temperatures from 25 degrees C to 28 degrees C with an optimum temperature of 28 degrees C. A partial monolayer was observed after 12 days in heart tissue. A primary culture was successfully obtained from heart tissue of C. chitala for the first time in India.

引用

页码：95 / 97

页数：3

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