Tumorigenic Cells Are Common in Mouse MPNSTs but Their Frequency Depends upon Tumor Genotype and Assay Conditions

被引：27
作者：
Buchstaller, Johanna [1 ,2 ]
McKeever, Paul E. [3 ]
Morrison, Sean J. [1 ,2 ,4 ]
机构：
[1] Univ Michigan, Dept Internal Med, Inst Life Sci, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA
[4] Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Childrens Res Inst, Dept Pediat, Dallas, TX 75390 USA
来源：
CANCER CELL | 2012年 / 21卷 / 02期
基金：
美国国家卫生研究院;
关键词：
HEMATOPOIETIC STEM-CELLS; ACUTE MYELOID-LEUKEMIA; INITIATING CELLS; MELANOMA-CELLS; SELF-RENEWAL; CANCER; IDENTIFICATION; MODEL; DIFFERENTIATION; EFFICIENT;
D O I：
10.1016/j.ccr.2011.12.027
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Tumor-initiating cells have been suggested to be rare in many cancers. We tested this in mouse malignant peripheral nerve sheath tumors (MPNSTs) and found that 18% of primary and 49% of passaged MPNST cells from Nfl(+/-); Ink4a/Arf(-/-) mice formed tumors upon transplantation, whereas only 1.8% to 2.6% of MPNST cells from Nf1(+/-); p53(+/-) mice did. MPNST cells of both genotypes require laminin binding to beta 1-integrin for clonogenic growth. Most MPNST cells from Nf1(+/-); Ink4a/Arf(-/-) mice expressed laminin, whereas most MPNST cells from Nf1(+/-); p53(+/-) mice did not. Exogenous laminin increased the percentage of MPNST cells from Nf1(+/-); p53(+/-) but not Nf1(+/-); Ink4a/Arf(-/-) mice that formed tumorigenic colonies. Tumor-forming potential is common among MPNST cells, but the assay conditions required to detect it vary with tumor genotype.
引用
收藏
页码：240 / 252
页数：13
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