Magnetic Silica Nanoparticle Cellular Uptake and Cytotoxicity Regulated by Electrostatic Polyelectrolytes-DNA Loading at Their Surface

被引：38

作者：

Davila-Ibanez, Ana B. ^{[1
]}

Salgueirino, Veronica ^{[1
]}

Martinez-Zorzano, Vicenta ^{[2
]}

Marino-Fernandez, Rosalia ^{[1
]}

Garcia-Lorenzo, Andres ^{[2
]}

Maceira-Campos, Melodie ^{[1
]}

Munoz-Ubeda, Monica ^{[3
]}

Junquera, Elena ^{[3
]}

Aicart, Emilio ^{[3
]}

Rivas, Jose ^{[4
]}

Javier Rodriguez-Berrocal, F. ^{[2
]}

Legido, Jose L. ^{[1
]}

机构：

[1] Univ Vigo, Dept Fis Aplicada, Vigo 36310, Spain

[2] Univ Vigo, Dept Bioquim Genet & Inmunol, Vigo 36310, Spain

[3] Univ Complutense Madrid, Dept Quim Fis, E-28040 Madrid, Spain

[4] Int Iberian Nanotechnol Lab, INL, P-4715330 Braga, Portugal

来源：

ACS NANO | 2012年 / 6卷 / 01期

关键词：

polyelectrolytes; DNA compaction; magnetoplexes; cellular uptake; cytotoxicity; CATIONIC LIPIDS; COBALT FERRITE; DELIVERY; COMPLEXES; SIZE; PARTICLES; SHELL; MULTILAYERS; TOXICITY; SPHERES;

D O I：

10.1021/nn204231g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Magnetic silica nanoparticles show great promise for drug delivery. The major advantages correspond to their magnetic nature and ease of biofunctionalization, which favors their ability to interact with cells and tissues. We have prepared magnetic silica nanoparticles with DNA fragments attached on their previously polyelectrolyte-primed surface. The remarkable feature of these materials is the compromise between the positive charges of the polyelectrolytes and the negative charges of the DNA. This dual-agent formulation dramatically changes the overall cytotoxicity and chemical degradation of the nanoparticles, revealing the key role that surface functionalization plays in regulating the mechanisms Involved.

引用

页码：747 / 759

页数：13

共 62 条

[1] New multivalent cationic lipids reveal bell curve for transfection efficiency versus membrane charge density: lipid-DNA complexes for gene delivery [J].