Silver nanoparticles induce developmental toxicity via oxidative stress and mitochondrial dysfunction in zebrafish (Danio rerio)

被引:40
作者
Lu, Chunjiao [1 ]
Lv, Yuhang [1 ]
Kou, Guanhua [1 ]
Liu, Yao [1 ]
Liu, Yi [1 ]
Chen, Yang [1 ]
Wu, Xuewei [1 ]
Yang, Fan [2 ]
Luo, Juanjuan [1 ,3 ]
Yang, Xiaojun [1 ]
机构
[1] Shantou Univ Med Coll, Guangdong Prov Key Lab Infect Dis & Mol Immunopath, Shantou, Peoples R China
[2] Shenzhen Ctr Dis Control & Prevent, Shenzhen, Peoples R China
[3] Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu, Peoples R China
基金
中国国家自然科学基金;
关键词
AgNPs; Mitochondrial dysfunction; Oxidative stress; Developmental toxicity; Zebrafish; DNA-DAMAGE; WATER; CELLS; STABILITY; APOPTOSIS; PATHWAY; INJURY; REPAIR; FATE; RATS;
D O I
10.1016/j.ecoenv.2022.113993
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Sliver nanoparticles (AgNPs) are widely used in industry, agriculture, and medicine, potentially resulting in adverse effects on human health and aquatic environments. Here, we investigated the developmental toxicity of zebrafish embryos with acute exposure to AgNPs. Our results demonstrated developmental defects in 4 hpf zebrafish embryos after exposure to different concentrations of AgNPs for 72 h. In addition, RNA-seq profiling of zebrafish embryos after AgNPs treatment. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the differentially expressed genes (DEGs) were enriched in DNA replication initiation, oxidoreductase activity, DNA replication, cellular senescence, and oxidative phos-phorylation signaling pathways in the AgNPs-treated group. Notably, we also found that AgNPs exposure could result in the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), the inhibition of su-peroxide dismutase (SOD), catalase (CAT), and mitochondrial complex I-V activities, and the downregulated expression of SOD, CAT, and mitochondrial complex I-IV chain-related genes. Moreover, the expression of mitochondrion-mediated apoptosis signaling pathway-related genes, such as bax, bcl2, caspase-3, and caspase-9, was significantly regulated after AgNPs exposure in zebrafish. Therefore, these findings demonstrated that AgNPs exposure could cause oxidative stress, induce mitochondrial dysfunction, and ultimately lead to developmental toxicity.
引用
收藏
页数:12
相关论文
共 68 条
[21]   Particulate and colloidal silver in sewage effluent and sludge discharged from British wastewater treatment plants [J].
Johnson, Andrew C. ;
Juergens, Monika D. ;
Lawlor, Alan J. ;
Cisowska, Iwona ;
Williams, Richard J. .
CHEMOSPHERE, 2014, 112 :49-55
[22]   Regulation of DNA damage tolerance in mammalian cells by post-translational modifications of PCNA [J].
Kanao, Rie ;
Masutani, Chikahide .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2017, 803 :82-88
[23]   The effect of biogenic manufactured silver nanoparticles on human endothelial cells and zebrafish model [J].
Khan, Imran ;
Bahuguna, Ashutosh ;
Krishnan, Manigandan ;
Shukla, Shruti ;
Lee, Hoomin ;
Mine, Sang-Hyun ;
Choi, Dong Kyu ;
Cho, Youngjin ;
Bajpai, Vivek K. ;
Huh, Yun Suk ;
Kang, Sun Chul .
SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 679 :365-377
[24]  
Kim H, 2021, AGING-US, V13, P19978, DOI 10.18632/aging.203419
[25]   Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues [J].
Kim, Soohee ;
Ryu, Doug-Young .
JOURNAL OF APPLIED TOXICOLOGY, 2013, 33 (02) :78-89
[26]  
Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/NMETH.1923, 10.1038/nmeth.1923]
[27]   Silver nanoparticle exposure impairs ion regulation in zebrafish embryos [J].
Lee, Chih-Ying ;
Horng, Jiun-Lin ;
Chen, Po-Yen ;
Lin, Li-Yih .
AQUATIC TOXICOLOGY, 2019, 214
[28]   A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination [J].
Lee, Dong-Hyun ;
Pan, Yunfeng ;
Kanner, Shlomo ;
Sung, Patrick ;
Borowiec, James A. ;
Chowdhury, Dipanjan .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2010, 17 (03) :365-U138
[29]   Toxic Effects and Mechanisms of Silver and Zinc Oxide Nanoparticles on Zebrafish Embryos in Aquatic Ecosystems [J].
Lee, Yen-Ling ;
Shih, Yung-Sheng ;
Chen, Zi-Yu ;
Cheng, Fong-Yu ;
Lu, Jing-Yu ;
Wu, Yuan-Hua ;
Wang, Ying-Jan .
NANOMATERIALS, 2022, 12 (04)
[30]   Environmental Transformations of Silver Nanoparticles: Impact on Stability and Toxicity [J].
Levard, Clement ;
Hotze, E. Matt ;
Lowry, Gregory V. ;
Brown, Gordon E., Jr. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2012, 46 (13) :6900-6914