Effects of hydration on the mechanical response of individual collagen fibrils

被引：117

作者：

Grant, Colin A. ^{[2
]}

Brockwell, David J. ^{[2
]}

Radford, Sheena E. ^{[2
]}

Thomson, Neil H. ^{[1
]}

机构：

[1] Univ Leeds, Sch Phys & Astron, Leeds LS2 9JT, W Yorkshire, England

[2] Univ Leeds, Inst Mol & Cellular Biol, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

APPLIED PHYSICS LETTERS | 2008年 / 92卷 / 23期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1063/1.2937001

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Collagen fibrils prepared from bovine Achilles tendon have been mechanically tested through nanoindentation by an atomic force microscope using force volume analysis. In ambient conditions where the fibrils are expected to be dehydrated, the elastic modulus was determined to be 1.9 +/- 0.5 GPa, while under aqueous fluid, it decreased by three orders of magnitude to 1.2 +/- 0.1 MPa. In air, fibril fracture occurred along the axis and the crack lengths were quantized to the D-banding periodicity. The apparent hardness of the fibrils was estimated to be in the range of 0.35-0.6 GPa. (C) 2008 American Institute of Physics.

引用

页数：3

共 24 条

[1] HYDRATION STRUCTURE OF A COLLAGEN PEPTIDE [J].

BELLA, J ;

BRODSKY, B ;

BERMAN, HM .

STRUCTURE, 1995, 3 (09) :893-906

[2] ATOMIC FORCE MICROSCOPE IMAGES OF COLLAGEN-FIBERS [J].

CHERNOFF, EAG ;

CHERNOFF, DA .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS, 1992, 10 (04) :596-599

[3] Measuring the elastic properties of thin polymer films with the atomic force microscope [J].

Domke, J ;

Radmacher, M .

LANGMUIR, 1998, 14 (12) :3320-3325

[4]

*EPAPS, EAPPLAB92081821 EPAP

[5] Poly(vinyl alcohol) hydrogel as a biocompatible viscoelastic mimetic for articular cartilage [J].

Grant, Colin ;

Twigg, Pete ;

Egan, Alex ;

Moody, Alexandra ;

Smith, Annie ;

Eagland, Donald ;

Crowther, Nicholas ;

Britland, Steve .

BIOTECHNOLOGY PROGRESS, 2006, 22 (05) :1400-1406

[6]

HASSAN EA, 1998, BIOPHYS J, V74, P1564

[7] Determination of the elastic modulus of native collagen fibrils via radial indentation [J].

Heim, August J. ;

Matthews, William G. ;

Koob, Thomas J. .

APPLIED PHYSICS LETTERS, 2006, 89 (18)

[8] Low strain nanomechanics of collagen fibrils [J].

Heim, August J. ;

Koob, Thomas J. ;

Matthews, William G. .

BIOMACROMOLECULES, 2007, 8 (11) :3298-3301

[9] Building collagen molecules, fibrils, and suprafibrillar structures [J].

Hulmes, DJS .

JOURNAL OF STRUCTURAL BIOLOGY, 2002, 137 (1-2) :2-10

[10] CALIBRATION OF ATOMIC-FORCE MICROSCOPE TIPS [J].

HUTTER, JL ;

BECHHOEFER, J .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1993, 64 (07) :1868-1873

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