MexXY efflux pump overexpression and aminoglycoside resistance in cystic fibrosis isolates of Pseudomonas aeruginosa from chronic infections

被引：29

作者：

Singh, Manu ^{[1
]}

Yau, Yvonne C. W. ^{[2
]}

Wang, Shirley ^{[1
]}

Waters, Valerie ^{[3
]}

Kumar, Ayush ^{[1
,4
]}

机构：

[1] Univ Manitoba, Dept Microbiol, Winnipeg, MB, Canada

[2] Hosp Sick Children, Div Microbiol, Dept Paediat Lab Med, Toronto, ON, Canada

[3] Hosp Sick Children, Div Infect Dis, Dept Paediat, Toronto, ON, Canada

[4] Univ Manitoba, Manitoba Chemosensory Biol Grp, Winnipeg, MB, Canada

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2017年 / 63卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

aminoglycoside resistance; MexZ; regulatory gene mutations; MULTIPLE ANTIBIOTIC-RESISTANCE; PORIN-ENCODING GENES; CLINICAL STRAINS; MEXAB-OPRM; MEXCD-OPRJ; MEXEF-OPRN; ANTIMICROBIAL SUSCEPTIBILITY; CANADIAN HOSPITALS; ACTIVE EFFLUX; EXPRESSION;

D O I：

10.1139/cjm-2017-0380

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we analyzed 15 multidrug-resistant cystic fibrosis isolates of Pseudomonas aeruginosa from chronic lung infections for expression of 4 different multidrug efflux systems (MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY), using quantitative reverse transcriptase PCR. Overexpression of MexXY pump was observed in all of the isolates tested. Analysis of regulatory genes that control the expression of these 4 efflux pumps revealed a number of previously uncharacterized mutations. Our work shows that MexXY pump overexpression is common in cystic fibrosis isolates and could be contributing to their reduced aminoglycoside susceptibility. Further, we also identified novel mutations in the regulatory genes of the 4 abovementioned Resistance-Nodulation-Division superfamily pumps that may be involved in the overexpression of these pumps.

引用

页码：929 / 938

页数：10

共 51 条

[1] Involvement of an active efflux system in the natural resistance of Pseudomonas aeruginosa to aminoglycosides [J].