Genetically Encoded Fluorescent Indicators for Organellar Calcium Imaging

被引：107

作者：

Suzuki, Junji ^{[1
,2
]}

Kanemaru, Kazunori ^{[1
]}

Iino, Masamitsu ^{[1
,3
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Pharmacol, Tokyo, Japan

[2] Univ Calif San Francisco, Dept Physiol, San Francisco, CA USA

[3] Nihon Univ, Sch Med, Dept Cellular & Mol Pharmacol, Tokyo, Japan

来源：

BIOPHYSICAL JOURNAL | 2016年 / 111卷 / 06期

关键词：

SARCOPLASMIC-RETICULUM CA2+; ENDOPLASMIC-RETICULUM; MITOCHONDRIAL CA2+; EXPANDED PALETTE; SKELETAL-MUSCLE; PRIMARY CILIUM; SINGLE-CELL; DYNAMICS; PROTEIN; SENSORS;

D O I：

10.1016/j.bpj.2016.04.054

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Optical Ca2+ indicators are powerful tools for investigating intracellular Ca2+ signals in living cells. Although a variety of Ca2+ indicators have been developed, deciphering the physiological functions and spatiotemporal dynamics of Ca2+ in intracellular organelles remains challenging. Genetically encoded Ca2+ indicators (GECIs) using fluorescent proteins are promising tools for organellar Ca2+ imaging, and much effort has been devoted to their development. In this review, we first discuss the key points of organellar Ca2+ imaging and summarize the requirements for optimal organellar Ca2+ indicators. Then, we highlight some of the recent advances in the engineering of fluorescent GECIs targeted to specific organelles. Finally, we discuss the limitations of currently available GECIs and the requirements for advancing the research on intraorganellar Ca2+ signaling.

引用

页码：1119 / 1131

页数：13

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