Characterization of an α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression

被引:14
作者
Carvallo, M
De Ioannes, P
Navarro, C
Chavez, R
Peirano, A
Bull, P
Eyzaguirre, J
机构
[1] Pontificia Univ Catolica Chile, Lab Bioquim, Dept Mol Genet & Microbiol, Santiago, Chile
[2] Univ Andres Bello, Escuela Artes Liberales, Santiago, Chile
来源
MYCOLOGICAL RESEARCH | 2003年 / 107卷
关键词
D O I
10.1017/S0953756203007603
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An alpha-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum was identified and sequenced. abf1 has an open reading frame of 1518 bp, does not contain introns and codes for a protein of 506 amino acids. The deduced mature protein has a molecular mass of 49.6 KDa, and its sequence is homologous to arabinofuranosidases of glycosyl hydrolase family 54. Southern blots suggest that abf1 is a single copy gene. Putative sequences for the binding of the transcriptional regulators X1nR, CreA, PacC, AlcR and AreA are present in the promoter. Northern-blot analysis shows that abf1 is expressed at neutral but not at alkaline or acidic pH values. The presence of binding sites for regulatory elements in the promoter region has been compared to the genes of other fungal enzymes belonging to the same family. This is the first characterization of an abf gene from a Penicillium species.
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页码:388 / 394
页数:7
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