The effect of gamma-ray irradiation on the fatty acid profile of chicken meat was examined at doses of 0.5, 2.5, 5.0, 7.5, 10.0, and 15.0 kGy by H-1-NMR spectroscopy. NMR spectral results revealed a dose-dependent effect of irradiation on the fatty acid profile. A trend toward an increase in the amount of saturated fatty acids and a decrease in the amount of polyunsaturated fatty acids in the triacylglycerol composition of the irradiated meat samples compared with the non-irradiated one was established with increasing the irradiation dose. The trend of decreasing polyunsaturated fatty acyl groups was associated with a decrease in oxidative stability of meat fat after application of gamma-ray irradiation.