Reversible immobilization of lysozyme via coupling to reversibly soluble polymer

被引：32
作者：
Chen, SH
Yen, YH
Wang, CL
Wang, SL [1 ]
机构：
[1] Da Yeh Univ, Dept Bioind Technol, Changhua 515, Taiwan
[2] Lan Yang Inst Technol, Dept Environm Engn, Ilan 261, Taiwan
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2003年 / 33卷 / 05期
关键词：
lysozyme; chitinase; reversibly soluble polymers; immobilized enzyme;
D O I：
10.1016/S0141-0229(03)00186-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Hen egg-white lysozyme (HEWL) can be covalently immobilized on a polymeric support (hydroxypropyl methylcellulose acetate succinate, AS-L), which is soluble above pH 5.5 but insoluble below pH 4.5. Commercial preparation of HEWL was used for the immobilization study. The immobilized enzyme (HEWL-AS) has much higher specific activity than those of free HEWL. The effects of pH and temperature on the activity and thermal stability of HEWL-AS were studied and compared with those of free HEWL. HEWL-AS retained nearly half of its initial chitinase activity even after heating at 70 degreesC for 50 min. The half-life at 4 degreesC was 4 days for free HEWL and 25 days for HEWL-AS. Following repeated pH cycles between 7 and 4.5, HEWL-AS retained nearly 50% of its original activity after 10 batches of chitinolytic reactions. (C) 2003 Elsevier Inc. All rights reserved.
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页码：643 / 649
页数：7
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