Effect of ppMCH derived peptides on PBMC proliferation and cytokine expression

被引:11
作者
Coumans, Bemard
Grisar, Thierry
Nahon, Jean-Louis
Lakaye, Bemard
机构
[1] Univ Liege, CNCM, B-4000 Cointe Ougree, Belgium
[2] Univ Nice, CNRS UMR 6097, Inst Pharmacol Mol & Cellulaire, Valbonne, France
基金
澳大利亚研究理事会;
关键词
prepro-MCH (ppMCH); melanin concentrating hon-none (MCH); neuropeptide gluramic acid-isoleucine (NEI); neuropeptide glycine-glutamic acid (NGE); peptide MCH-NEl; peripheral blood mononuclear cell (PBMC); cytokines;
D O I
10.1016/j.regpep.2007.04.001
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The mRNA encoding prepro-Melanin concentrating honnone (ppMCH) is mainly expressed in the central nervous system but has also been detected at lower amount in many peripheral tissues including spleen and thymus. At the peptide level however, several forms of the precursor can be detected in these tissues and are sometimes expressed at similar levels compared to brain. In the present work, we have studied the in vitro action of a wide range of concentration (1 nM to 1 mu M) of the different peptides encoded by ppMCH i.e. neuropeptide glycine-glutamic acid (NGE), neuropeptide glutamic acid-isoleucine (NEI), Melanin concentrating hormone (MCH) and the dipeptide NEI-MCH on peripheral blood mononuclear cells (PBMC) proliferation and cytokine production following anti-CD3 stimulation. Among them only MCH decreased PBMC proliferation with a maximal effect of 35% at 100 nM. Moreover as demonstrated by using ELISA, MCH significantly decreases IL-2 production by 25% but not IL-4, INF-gamma or TNF-alpha expression. Interestingly, exogenous IL-2 decreases significantly MCH-mediated inhibition, suggesting that it is an important downstream mediator of MCH action. Finally, we showed that after 7 to 9 days of incubation, MCH also inhibits proliferation of non-stimulated PBMC. Altogether, these data demonstrate that fully mature MCH modulates proliferation of anti-CD3 stimulated PBMC partially through regulation of IL-2 production. (c) 2007 Elsevier B.V All rights reserved.
引用
收藏
页码:104 / 108
页数:5
相关论文
共 25 条
  • [11] PRO-MELANIN CONCENTRATING HORMONE MESSENGER-RIBONUCLEIC-ACID AND PEPTIDES EXPRESSION IN PERIPHERAL-TISSUES OF THE RAT
    HERVIEU, G
    NAHON, JL
    [J]. NEUROENDOCRINOLOGY, 1995, 61 (04) : 348 - 364
  • [12] IWAZ J, 1986, CELL IMMUNOL, V102, P455
  • [13] Melanin-concentrating hormone signaling systems in fish
    Kawauchi, H
    Baker, BI
    [J]. PEPTIDES, 2004, 25 (10) : 1577 - 1584
  • [14] CHARACTERIZATION OF MELANIN-CONCENTRATING HORMONE IN CHUM SALMON PITUITARIES
    KAWAUCHI, H
    KAWAZOE, I
    TSUBOKAWA, M
    KISHIDA, M
    BAKER, BI
    [J]. NATURE, 1983, 305 (5932) : 321 - 323
  • [15] Peptides as regulators of the immune system: emphasis on somatostatin
    Krantic, S
    [J]. PEPTIDES, 2000, 21 (12) : 1941 - 1964
  • [16] Promoter characterization of the mouse melanin-concentrating hormone receptor 1
    Lakaye, B
    Adamantidis, A
    Coumans, B
    Grisar, T
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2004, 1678 (01): : 1 - 6
  • [17] Neuropeptides, by direct interaction with T cells, induce cytokine secretion and break the commitment to a distinct T helper phenotype
    Levite, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) : 12544 - 12549
  • [18] Appetite-boosting property of pro-melanin-concentrating hormone131-165 (neuropeptide-glutamic acid-isoleucine) is associated with proteolytic resistance
    Maulon-Feraille, L
    Della Zuana, O
    Suply, T
    Rovere-Jovene, C
    Audinot, V
    Levens, N
    Boutin, JA
    Duhault, J
    Nahon, JL
    [J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2002, 302 (02) : 766 - 773
  • [19] Age-related changes in the neuropeptide Y effects on murine lymphoproliferation and interleukin-2 production
    Medina, S
    Del Río, M
    Hernanz, A
    De la Fuente, M
    [J]. PEPTIDES, 2000, 21 (09) : 1403 - 1409
  • [20] Melanin-concentrating hormone (MCH) antagonizes the effects of alpha-MSH and neuropeptide E-I on grooming and locomotor activities in the rat
    Sanchez, M
    Baker, BI
    Celis, M
    [J]. PEPTIDES, 1997, 18 (03) : 393 - 396