Cylindrical internal reflectance FT-IR interactions between components of biological molecules and metal ions - II

The interactions of metal ions with D-ribose and 2'-D-deoxyribose as weil as 2'-D-deoxyadenosine and 2'-D-deoxyadenosine-5'-monophosphate have been investigated in solid, glassy state and in water solutions by comparing Diffuse Internal Reflectance, Cylindrical Internal Reflectance and Transmittance spectra. The metal ion mainly interacts with C-C, C-O bonds of sugars as well as with N(I)=C-NH2, N(7) and phosphate moieties of nucleotide. Cylindrical Reflectance, even in dilute solutions, constitutes an useful technique to study the behaviour of biological molecule fragments in water.