Nonoptically probing near-field microscopy for the observation of biological living specimens

被引：12

作者：

Kawata, Y ^{[1
]}

Murakami, M

Egami, C

Sugihara, O

Okamoto, N

Tsuchimori, M

Watanabe, O

Nakamura, O

机构：

[1] Shizuoka Univ, Fac Engn, Joho Ku, Hamamatsu, Shizuoka 4328581, Japan

[2] Toyota Cent Res & Dev Labs Inc, Aichi 4801192, Japan

[3] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan

来源：

APPLIED PHYSICS LETTERS | 2001年 / 78卷 / 15期

关键词：

D O I：

10.1063/1.1350965

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We present the observation of living specimens with subwavelength resolution by using the nonoptically probing near-field microscopy we have developed recently. In the near-field microscope, the optical field distributions near the specimens are recorded as the surface topography of a photosensitive film, and the topographical distributions are readout with an atomic-force microscopy. Since the near-field microscope does not require the scanning of a probe tip for illumination or detection or scattering of light, it is possible to observe moving biological specimens and fast phenomena. We demonstrate the observation of a moving paramecium and euglena gracilis with subwavelength resolution. The observation of the nucleus inside a euglena cell was also demonstrated. (C) 2001 American Institute of Physics.

引用

页码：2247 / 2249

页数：3

共 13 条

[1] NEAR-FIELD SCANNING OPTICAL MICROSCOPY (NSOM) - DEVELOPMENT AND BIOPHYSICAL APPLICATIONS [J].

BETZIG, E ;

LEWIS, A ;

HAROOTUNIAN, A ;

ISAACSON, M ;

KRATSCHMER, E .

BIOPHYSICAL JOURNAL, 1986, 49 (01) :269-279

[2] NEAR-FIELD OPTICS - MICROSCOPY WITH NANOMETER-SIZE FIELDS [J].

DENK, W ;

POHL, DW .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (02) :510-513

[3] SUB-MICROSCOPIC PATTERN REPLICATION WITH VISIBLE-LIGHT [J].

FISCHER, UC ;

ZINGSHEIM, HP .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY, 1981, 19 (04) :881-885

[4] NEAR-FIELD SPECTROSCOPY OF THE QUANTUM CONSTITUENTS OF A LUMINESCENT SYSTEM [J].

HESS, HF ;

BETZIG, E ;

HARRIS, TD ;

PFEIFFER, LN ;

WEST, KW .

SCIENCE, 1994, 264 (5166) :1740-1745

[5] NEAR-FIELD SCANNING OPTICAL MICROSCOPE WITH A METALLIC PROBE TIP [J].

INOUYE, Y ;

KAWATA, S .

OPTICS LETTERS, 1994, 19 (03) :159-161

[6] Three-dimensional optical data storage using photochromic materials [J].

Kawata, S ;

Kawata, Y .

CHEMICAL REVIEWS, 2000, 100 (05) :1777-1788

[7] Use of two-photon absorption in a photorefractive crystal for three-dimensional optical memory [J].

Kawata, Y ;

Ishitobi, H ;

Kawata, S .

OPTICS LETTERS, 1998, 23 (10) :756-758

[8] Feasibility of molecular-resolution fluorescence near-field microscopy using multi-photon absorption and field enhancement near a sharp tip [J].

Kawata, Y ;

Xu, C ;

Denk, W .

JOURNAL OF APPLIED PHYSICS, 1999, 85 (03) :1294-1301

[9] Non-optically probing near-field microscopy [J].

Kawata, Y ;

Egami, C ;

Nakamura, O ;

Sugihara, O ;

Okamoto, N ;

Tsuchimori, M ;

Watanabe, O .

OPTICS COMMUNICATIONS, 1999, 161 (1-3) :6-12

[10] Polarized laser induced holographic surface relief gratings on polymer films [J].

Kim, DY ;

Li, L ;

Jiang, XL ;

Shivshankar, V ;

Kumar, J ;

Tripathy, SK .

MACROMOLECULES, 1995, 28 (26) :8835-8839

← 1 2 →