Exploring biomolecular interactions by single-molecule fluorescence

被引：16
|
作者：
Pappas, Dimitri [1 ]
Burrows, Sean M. [1 ]
Reif, Randall D. [1 ]
机构：
[1] Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2007年 / 26卷 / 09期
关键词：
fluorescence anisotropy; fluorescence spectroscopy; lifetime; fluorescence (Forster) resonance-energy transfer; FRET; single cell; single molecule;
D O I：
10.1016/j.trac.2007.09.003
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：884 / 894
页数：11
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