High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology

被引：1

作者：

Lomakin, Ya A. ^{[1
,2
]}

Kaminskaya, A. N. ^{[1
]}

Stepanov, A., V ^{[1
,2
]}

Shmidt, A. A. ^{[1
]}

Gabibov, A. G. ^{[1
,3
]}

Belogurov, A. A., Jr. ^{[1
,3
]}

机构：

[1] Russian Acad Sci, MM Shemyakin & Yu A Ovchinnikov Inst Bioorgan Che, Moscow, Russia

[2] Kazan Volga Reg Fed Univ, Kazan, Russia

[3] Moscow MV Lomonosov State Univ, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2019年 / 167卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

M13K07; bacteriophage; B-cell receptor; phage display; follicular lymphoma; flow cytofluorometry; LYMPHOMA;

D O I：

10.1007/s10517-019-04546-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.

引用

页码：446 / 451

页数：6

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