Electrogenetics: Bridging synthetic biology and electronics to remotely control the behavior of mammalian designer cells

被引：15

作者：

Mansouri, Maysam ^{[1
]}

Fussenegger, Martin ^{[1
,2
]}

机构：

[1] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, Mattenstr 26, CH-4058 Basel, Switzerland

[2] Univ Basel, Fac Sci, Mattenstr 26, CH-4058 Basel, Switzerland

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2022年 / 68卷

基金：

欧洲研究理事会;

关键词：

Synthetic biology; Therapeutic cell engineering; Traceless switch control; Electrostimulation; Electrogenetics; GENE;

D O I：

10.1016/j.cbpa.2022.102151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrogenetics, the combination of electronics and genetics, is an emerging field of mammalian synthetic biology in which electrostimulation is used to remotely program user-designed genetic elements within designer cells to generate desired outputs. Here, we describe recent advances in electro-induced therapeutic gene expression and therapeutic protein secretion in engineered mammalian cells. We also review available tools and strategies to engineer electro-sensitive therapeutic designer cells that are able to sense electrical pulses and produce appropriate clinically relevant outputs in response. We highlight current limitations facing mammalian electrogenetics and suggest potential future directions for research.

引用

页数：6

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