Investigation of ischemia damaging action on blood serum by Raman spectroscopy method

被引:15
作者
Baranov, AN [1 ]
Vlasova, L [1 ]
Saletsky, AM [1 ]
机构
[1] Moscow MV Lomonosov State Univ, Dept Phys, Moscow 119992, Russia
关键词
Raman spectroscopy; blood serum; lipoprotein; free radicals;
D O I
10.1002/lapl.200410124
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Raman spectroscopy method is applied for analysis of ischemia damaging action on serum. The registered changes of low-density lipoprotein (LDL) after brain ischemia (size increasing, density reducing) are explained in frameworks of free radical theory of oxidative stress. We confirmed by Raman spectroscopy method our hypothesis, according to which the increase in LDL size and decrease of LDL density after an ischemia, in contrast to LDL of healthy animals, is caused by loosing of LDL phospholipid monolayer under damaging action of free radicals. The obtained results show the possibility of using of Raman spectroscopy method for an estimation of ischemic damages.
引用
收藏
页码:555 / 559
页数:5
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