Lrig1 marks a population of gastric epithelial cells capable of long-term tissue maintenance and growth in vitro

被引:15
作者
Schweiger, Pawel J. [1 ]
Clement, Ditte L. [1 ]
Page, Mahalia E. [2 ]
Schepeler, Troels [1 ]
Zou, Xiangang [4 ]
Sirokmany, Gabor [2 ,6 ]
Watt, Fiona M. [3 ]
Jensen, Kim B. [1 ,5 ]
机构
[1] Univ Copenhagen, BRIC, DK-2200 Copenhagen N, Denmark
[2] Cambridge Stem Cell Inst, MRC, Wellcome Trust, Tennis Court Rd, Cambridge CB2 1QR, England
[3] Kings Coll London, Ctr Stem Cells & Regenerat Med, 28th Floor,Tower Wing Guys Campus, London SE1 9RT, England
[4] Canc Res UK Cambridge Inst, Cambridge CB2 0RE, England
[5] Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Stem Cell Biol, DK-2200 Copenhagen N, Denmark
[6] Semmelweis Univ, Dept Physiol, Tuzolto St 37-47, H-1094 Budapest, Hungary
来源
SCIENTIFIC REPORTS | 2018年 / 8卷
基金
英国医学研究理事会;
关键词
STEM-CELLS; CHIEF CELLS; STOMACH; PROGENITOR; METAPLASIA; CANCER; IDENTIFICATION; ACTIVATION; REGULATOR; EXPANSION;
D O I
10.1038/s41598-018-33578-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The processes involved in renewal of the epithelium that lines the mouse stomach remain unclear. Apart from the cells in the isthmus, several other populations located deeper in the gastric glands have been suggested to contribute to the maintenance of the gastric epithelium. Here, we reveal that Lrig1 is expressed in the basal layer of the forestomach and the lower part of glands in the corpus and pylorus. In the glandular epithelium of the stomach, Lrig1 marks a heterogeneous population comprising mainly non-proliferative cells. Yet, fate-mapping experiments using a knock-in mouse line expressing Cre specifically in Lrig1(+) cells demonstrate that these cells are able to contribute to the long-term maintenance of the gastric epithelium. Moreover, when cultured in vitro, cells expressing high level of Lrig1 have much higher organoid forming potential than the corresponding cellular populations expressing lower levels of Lrig1. Taken together, these observations show that Lrig1 is expressed primarily by differentiated cells, but that these cells can be recruited to contribute to the maintenance of the gastric epithelium. This confirms previous observations that cells located in the lower segments of gastric glands can participate in tissue replenishment.
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页数:11
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