Isolation and genetic characterization of Toxoplasma gondii from raccoons (Procyon lotor), cats (Felis domesticus), striped skunk (Mephitis mephitis), black bear (Ursus americanus), and cougar (Puma concolor) from Canada

被引：41

作者：

Dubey, J. R. ^{[1
]}

Quirk, T. ^{[2
]}

Pitt, J. A. ^{[3
]}

Sundar, N. ^{[1
]}

Velmurugan, G. V. ^{[1
]}

Kwok, O. C. H. ^{[1
]}

Leclair, D. ^{[4
]}

Hill, R. ^{[5
]}

Su, C. ^{[5
]}

机构：

[1] USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Agr Res Serv, Beltsville, MD 20705 USA

[2] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada

[3] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

[4] Makivik Corp, Nunavik Res Ctr, Kuujjuaq, PQ JOM 1MO, Canada

[5] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA

来源：

JOURNAL OF PARASITOLOGY | 2008年 / 94卷 / 01期

关键词：

D O I：

10.1645/GE-1349.1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Viable Toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats (Felis domesticus), 2 raccoons (Procyon lotor), a skunk (Mephitis mephitis) trapped in remote locations in Manitoba, Canada, and a black bear (Ursus americanus) from Kuujjuaq, northern Quebec, Canada. Genotyping of these T. gondii isolates using polymorphisms at 10 nuclear markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast market Apico revealed 4 genotypes. None of the isolates was clonal archetypal Types I, II, and III found in the United States. These results are in contrast with the Type It genotype that is widespread in domestic animals and humans throughout the United States and Europe. This is the first genotyping of T. gondii isolates from this part of North America.

引用

页码：42 / 45

页数：4

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