Novel photocleavable universal support for oligonueleotide synthesis

被引：5

作者：

Anderson, E

Brown, T

Picken, D

机构：

[1] Link Technol Ltd, Bellshill ML4 3BF, Lanark, Scotland

[2] Univ Southampton, Dept Chem, Southampton SO9 5NH, Hants, England

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

photocleavable support; universal support; oligonucleotide synthesis;

D O I：

10.1081/NCN-120022996

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel photocleavable universal support for the automated solid phase synthesis of oligonucleotides is described. The linker between the growing oligonucleotide chain and CPG support contains a nucleophilic amine protected with a photocleavable group. On exposure to UV light, this group is detached and the free amine affords cleavage of the oligonucleotide from the support. The use of long wavelength UV light avoids damage to the DNA.

引用

页码：1403 / 1406

页数：4

共 3 条

[1] Linkers and cleavage strategies in solid-phase organic synthesis and combinatorial chemistry [J].