Carbon nanodots combined with loop-mediated isothermal amplification (LAMP) for detection of African swine fever virus (ASFV)

被引：17

作者：

Cao, Gaihua ^{[1
]}

Qiu, Yue ^{[1
]}

Long, Keyi ^{[1
]}

Xiong, Yifan ^{[1
]}

MeimeiShi ^{[2
]}

JunYang ^{[2
]}

Li, Yingguo ^{[2
]}

Nie, Fuping ^{[2
]}

Huo, Danqun ^{[1
,3
]}

Hou, Changjun ^{[1
]}

机构：

[1] Chongqing Univ, Key Lab Biorheol Sci & Technol, Bioengn Coll, Minist Educ, Chongqing 400044, Peoples R China

[2] Chongqing Customs Technol Ctr, State Key Lab Cattle Dis Detect Chongqing, Chongqing 400020, Peoples R China

[3] Chongqing Univ, Sch Microelect & Commun Engn, Chongqing Key Lab Biopercept & Intelligent Inform, Chongqing 400044, Peoples R China

来源：

MICROCHIMICA ACTA | 2022年 / 189卷 / 09期

基金：

中国国家自然科学基金;

关键词：

African swine fever virus (ASFV); Carbon nanodot; Loop-mediated isothermal amplification (LAMP); Fluorescence; RAPID DETECTION; FLUORESCENT;

D O I：

10.1007/s00604-022-05390-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The spread of African swine fever virus (ASFV) caused huge economic costs, so early detection is particularly important. Here, we established a fluorescence biosensor based on carbon nanodots (CNDs) and loop-mediated isothermal amplification (LAMP) to ultra-sensitively detect ASFV. LAMP with high efficiency produced a large amount of pyro phosphoric acid and caused pH change in a short time. CNDs with strong light stability had a large fluorescence response at the emission wavelength of 585.5 nm to small pH change by the excitation wavelength of 550 nm. The biosensor realized "turn-off-on" mode for ASFV detection with the detection limit as low as 15.21 copies mu L-1. In addition, the biosensor had high accuracy in the actual sample assay. Therefore, the biosensor achieved rapid, sensitive, low-cost, and simple detection for ASFV. Moreover, the biosensor broadened the detection pathway of LAMP as a tool with great development prospect.

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页数：9

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