Identifying Specific and Differentially Linked Glycosyl Residues in Mammalian Glycans by Targeted LC-MS Analysis

被引：6

作者：

Huang, Yi-Li ^{[1
]}

Jhou, Bing-Yi ^{[1
]}

Chen, Sung-Fang ^{[1
]}

Khoo, Kay-Hooi ^{[2
]}

机构：

[1] Natl Taiwan Normal Univ, Dept Chem, Taipei 11677, Taiwan

[2] Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan

来源：

ANALYTICAL SCIENCES | 2018年 / 34卷 / 09期

关键词：

Linkage analysis; partially methylated alditol; LC-MS; MASS-SPECTROMETRIC ANALYSIS;

D O I：

10.2116/analsci.18SCP01

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds. Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest. In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported. N-Glycans were first per methylated with methyl iodide, and the levels of methylation were further confirmed by MALDI-TOF. PMAs were then produced via complete hydrolysis and reduction. PMAs derived from Fetuin N-glycan and Lewisa antigen carbohydrates were successfully detected by LC-MS analysis. This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.

引用

页码：1049 / 1054

页数：6

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