Assessing biomarkers of oxidative stress: Analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection

被引：27

作者：

Bolin, Celeste ^{[1
]}

Cardozo-Pelaez, Fernando ^{[1
]}

机构：

[1] Univ Montana, Dept Pharmaceut Sci, Environm Hlth Sci Ctr, Missoula, MT 59812 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 856卷 / 1-2期

关键词：

nucleotide triphosphates; HPLC-EC; oxidized nucleotides; GTP; oxidative stress; biomarker;

D O I：

10.1016/j.jchromb.2007.05.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidation of the guanosine moiety in DNA has become a hallmark biomarker in assessing oxidative stress. The oxidation of guanosine in the nucleotide triphosphate pool has been overlooked due to the lack of a reliable methodology. This method describes a sample processing and high performance liquid chromatography with electrochemical detection protocol for the analysis of the cellular pool of guanosine triphosphates and oxidized guanosine triphosphates. Validation of this method is demonstrated along with evaluation of these analytes in control and oxidizing conditions in vitro and in HEK 293T cells. Oxidation of this triphosphate pool occurred independently of oxidation to DNA. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：121 / 130

页数：10

共 39 条

[1] Oxidative DNA damage in the parkinsonian brain: An apparent selective increase in 8-hydroxyguanine levels in substantia nigra
Alam, ZI
Jenner, A
Daniel, SE
Lees, AJ
Cairns, N
Marsden, CD
Jenner, P
Halliwell, B
[J]. JOURNAL OF NEUROCHEMISTRY, 1997, 69 (03) : 1196 - 1203
[2] Mycophenolate mofetil and its mechanisms of action
Allison, AC
Eugui, EM
[J]. IMMUNOPHARMACOLOGY, 2000, 47 (2-3): : 85 - 118
[3] Beal MF, 2003, ANN NY ACAD SCI, V991, P120
[4] Role of guanosine triphosphate in ferric ion-linked Fenton chemistry
Biaglow, JE
Held, KD
Manevich, Y
Tuttle, S
Kachur, A
Uckun, F
[J]. RADIATION RESEARCH, 1996, 145 (05) : 554 - 562
[5] Single extraction protocol for the analysis of 8-hydroxy-2′-deoxyguanosine (oxo8dG) and the associated activity of 8-oxoguanine DNA glycosylase
Bolin, C
Stedeford, T
Cardozo-Pelaez, F
[J]. JOURNAL OF NEUROSCIENCE METHODS, 2004, 136 (01) : 69 - 76
[6] THE PECKING ORDER OF FREE-RADICALS AND ANTIOXIDANTS - LIPID-PEROXIDATION, ALPHA-TOCOPHEROL, AND ASCORBATE
BUETTNER, GR
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (02) : 535 - 543
[7] CHENG KC, 1992, J BIOL CHEM, V267, P166
[8] Urinary 8-hydroxydeoxyguano sine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers
Chiou, CC
Chang, PY
Chan, EC
Wu, TL
Tsao, KC
Wu, JT
[J]. CLINICA CHIMICA ACTA, 2003, 334 (1-2) : 87 - 94
[9] COHEN MB, 1981, J BIOL CHEM, V256, P8713
[10] Oxidative damage to DNA in diabetes mellitus
Dandona, P
Thusu, K
Cook, S
Snyder, B
Makowski, J
Armstrong, D
Nicotera, T
[J]. LANCET, 1996, 347 (8999) : 444 - 445

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