Genetic and molecular organization of the alkylbenzene catabolism operon in the psychrotrophic strain Pseudomonas putida 01G3

被引：14

作者：

Chablain, PA

Zgoda, AL

Sarde, CO

Truffaut, N

机构：

[1] Univ Technol Compiegne, UMR 6022 CNRS, Lab Genet Microbienne, F-60205 Compiegne, France

[2] Univ Technol Compiegne, UMR 6022 CNRS, Technol Enzymat Lab, F-60205 Compiegne, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 01期

关键词：

D O I：

10.1128/AEM.67.1.453-458.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Il-kb sequence encompassing the alkylbenzene upper pathway in Pseudomonas putida 01G3, a psychrotrophic strain able to degrade alkylbenzenes at low temperatures, was characterized. Together with a potential regulator (EbdR), six putative enzymes (EbdAaAbAcAdBC) were identified, and they exhibited highly significant similarities with enzymes implicated in the equivalent pathway in P. putida RE204. ebd genes appeared to be preferentially induced by ethylbenzene. Multiple-alignment data and growth rate measurements led us to classify 01G3 and closely related strains in two groups with distinct substrate specificities. Close to identified genes, remnants of ISS-like elements provided insight into the evolution of catabolic sequences through rearrangements fi om a less complex ancestral cluster.

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页码：453 / 458

页数：6

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