Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

被引：12

作者：

De Nes, Fernanda ^{[1
]}

Riboldi, Gustavo Pelicioli ^{[2
]}

Guedes Frazzon, Ana Paula ^{[3
]}

d'Azevedo, Pedro Alves ^{[4
]}

Frazzon, Jeverson ^{[1
,2
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICTA, Grad Program Food Sci & Technol, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Ctr Biotechnol, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Dept Microbiol, Porto Alegre, RS, Brazil

[4] Fed Fdn Med Sci Porto Alegre, Dept Microbiol, Porto Alegre, RS, Brazil

来源：

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL | 2010年 / 43卷 / 04期

关键词：

Listeria monocytogenes; PCR-REA; RAPD; Dairy products; PREVALENCE; STRAINS; GENES; RAPD;

D O I：

10.1590/S0037-86822010000400009

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Introduction: Listeria monocytogenes is a ubiquitous microorganism in nature and is responsible for listeriosis, an infectious disease caused by consumption of contaminated food. Methods: Molecular characterization was performed on 19 strains of Listeria monocytogenes (serovars 1/2a, 1/2b, 4b and 4c), isolated from dairy products in Rio Grande do Sul, Brazil. The molecular techniques applied were random amplification of polymorphic DNA and restriction enzyme analysis. In addition to the molecular analysis, the antimicrobial resistance profile was determined. Results: The strains studied showed a low degree of diversity. In relation to the antimicrobial resistance profile of those microorganisms from the samples analyzed, all of them were susceptible to the antimicrobials tested. Conclusions: The molecular techniques that were used presented good discriminatory power for the strains studied. Furthermore, all of the samples that were analyzed were susceptible to the antimicrobials tested.

引用

页码：382 / 385

页数：4

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