Molecular and immunological characterization of novel weed pollen pan-allergens

被引：49

作者：

Wopfner, N. ^{[1
]}

Gruber, P. ^{[1
]}

Wallner, M. ^{[1
]}

Briza, P. ^{[1
]}

Ebner, C. ^{[2
]}

Mari, A. ^{[3
]}

Richter, K. ^{[4
]}

Vogel, L. ^{[5
]}

Ferreira, F. ^{[1
]}

机构：

[1] Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, Dept Mol Biol, A-5020 Salzburg, Austria

[2] Allergieambulatorium, Vienna, Austria

[3] IDI IRCCS, Ctr Clin Expt Allergol, Rome, Italy

[4] Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

[5] Paul Ehrlich Inst, Dept Allergol, D-6070 Langen, Germany

来源：

ALLERGY | 2008年 / 63卷 / 07期

关键词：

calcium-binding proteins; cross-reactivity; mugwort pollen; pan-allergens; profilin; ragweed pollen;

D O I：

10.1111/j.1398-9995.2008.01635.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Pan-allergens like profilins, calcium-binding proteins (CBPs), and nonspecific lipid transfer proteins have been suggested as possible specific markers for multiple pollen sensitizations, and could be used to predict cross-sensitization/poly-sensitization to several pollen allergens. Therefore, the purification and characterization of cross-reacting allergens in pollen is an extremely important task towards correct allergy diagnosis. Methods: New pan-allergens were identified by screening a ragweed pollen cDNA library with sera of patients allergic to mugwort pollen. Resulting proteins were cloned, expressed, purified and characterized. Results: We report complete cDNA sequences of two profilin isoforms (Amb a 8.01 and Amb a 8.02), two isoforms of a 2EF-hand CBP (Amb a 9.01 and Amb a 9.02), a new 3EF-hand CBP (Amb a 10) from ragweed pollen and a 2EF-hand CBP from mugwort (Art v 5). All these proteins were expressed in Escherichia coli, purified to homogeneity and characterized by biochemical and immunological means. Conclusions: The identified proteins are novel pan-allergens and can be used as diagnostic markers for polysensitization and used in component-resolved diagnosis.

引用

页码：872 / 881

页数：10

共 28 条

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