Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

被引：33

作者：

Li, Yan ^{[1
]}

Wong, Ying Lei ^{[1
]}

Lee, Michelle Yueqi ^{[1
]}

Li, Qingxin ^{[2
]}

Wang, Qing-Yin ^{[3
]}

Lescar, Julien ^{[4
]}

Shi, Pei-Yong ^{[5
,6
]}

Kang, CongBao ^{[1
]}

机构：

[1] ASTAR, Ctr Expt Therapeut, 31 Biopolis Way,Nanos 03-01, Singapore 138669, Singapore

[2] Agcy Sci Technol & Res, Inst Chem & Engn Sci, 1 Pesek Rd, Singapore 627833, Singapore

[3] Novartis Inst Trop Dis, Singapore, Singapore

[4] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore

[5] Univ Texas Med Branch, Dept Pharmacol & Toxicol, Dept Biochem & Mol Biol, Galveston, TX 77555 USA

[6] Univ Texas Med Branch, Sealy Ctr Struct Biol & Mol Biophys, Galveston, TX 77555 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 39期

关键词：

dengue virus; membrane protein; membrane topology; NMR spectroscopy; NS4B; NONSTRUCTURAL PROTEIN NS2A; NMR CHEMICAL-SHIFTS; NS3; COMPLEX; SPECTROSCOPY; ASSIGNMENT; HELICASE; DOMAIN; INHIBITORS; MUTATIONS;

D O I：

10.1002/anie.201606609

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.

引用

页码：12068 / 12072

页数：5

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