Detection of West Nile virus lineages 1 and 2 by real-time PCR

被引：115

作者：

Linke, Sonja ^{[1
]}

Ellerbrok, Heinz ^{[1
]}

Niedrig, Matthias ^{[1
]}

Nitsche, Andreas ^{[1
]}

Pauli, Georg ^{[1
]}

机构：

[1] Robert Koch Inst, Zentrum Biol Sicherheit, D-13353 Berlin, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 2007年 / 146卷 / 1-2期

关键词：

West Nile virus; genotype lineages 1 and 2; Kunjin virus; real-time RT-PCR;

D O I：

10.1016/j.jviromet.2007.05.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

West Nile virus (WNV) is a Flavivirus attracting worldwide attention because it has spread rapidly across the Americas since its first appearance in New York City in 1999. Several PCR-based diagnostic methods have been developed for the detection of WNV. The focus of these assays has been WNV lineage 1 which can be found worldwide, while lineage 2 viruses were thought to be endemic only in some regions of Africa. However, both lineages may be imported from Africa to Europe by migrating birds. In order to determine the incidence of WNV in Germany, a real-time-based PCR assay was developed, targeting a conserved region of WNV lineages 1 and 2. This assay is a suitable tool for the diagnosis of WNV and for surveillance studies. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：355 / 358

页数：4

共 17 条

[1]

Bakonyi T, 2006, EMERG INFECT DIS, V12, P618

[2] Novel flavivirus or new lineage of West Nile Virus, central Europe [J].

Bakonyi, T ;

Hubálek, Z ;

Rudolf, I ;

Nowotny, N .

EMERGING INFECTIOUS DISEASES, 2005, 11 (02) :225-231

[3] Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses [J].

Berthet, FX ;

Zeller, HG ;

Drouet, MT ;

Rauzier, J ;

Digoutte, JP ;

Deubel, V .

JOURNAL OF GENERAL VIROLOGY, 1997, 78 :2293-2297

[4] Identification of a Kunjin/West Nile-like flavivirus in brains of patients with New York encephalitis [J].

Briese, T ;

Jia, XY ;

Huang, C ;

Grady, LJ ;

Lipkin, WI .

LANCET, 1999, 354 (9186) :1261-1262

[5] Phylogenetic relationships of southern African West Nile virus isolates [J].

Burt, FJ ;

Grobbelaar, AA ;

Leman, PA ;

Anthony, FS ;

Gibson, GVF ;

Swanepoel, R .

EMERGING INFECTIOUS DISEASES, 2002, 8 (08) :820-826

[6] West Nile virus: Epidemiology and clinical features of an emerging epidemic in the United States [J].

Hayes, EB ;

Gubler, DJ .

ANNUAL REVIEW OF MEDICINE, 2006, 57 :181-194

[7]

Hubálek Z, 1999, EMERG INFECT DIS, V5, P643, DOI 10.3201/eid0505.990505

[8] Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay [J].

Lanciotti, RS ;

Kerst, AJ ;

Nasci, RS ;

Godsey, MS ;

Mitchell, CJ ;

Savage, HM ;

Komar, N ;

Panella, NA ;

Allen, BC ;

Volpe, KE ;

Davis, BS ;

Roehrig, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4066-4071

[9] Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle East [J].

Lanciotti, RS ;

Ebel, GD ;

Deubel, V ;

Kerst, AJ ;

Murri, S ;

Meyer, R ;

Bowen, M ;

McKinney, N ;

Morrill, WE ;

Crabtree, MB ;

Kramer, LD ;

Roehrig, JT .

VIROLOGY, 2002, 298 (01) :96-105

[10] Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States [J].

Lanciotti, RS ;

Roehrig, JT ;

Deubel, V ;

Smith, J ;

Parker, M ;

Steele, K ;

Crise, B ;

Volpe, KE ;

Crabtree, MB ;

Scherret, JH ;

Hall, RA ;

MacKenzie, JS ;

Cropp, CB ;

Panigrahy, B ;

Ostlund, E ;

Schmitt, B ;

Malkinson, M ;

Banet, C ;

Weissman, J ;

Komar, N ;

Savage, HM ;

Stone, W ;

McNamara, T ;

Gubler, DJ .

SCIENCE, 1999, 286 (5448) :2333-2337

← 1 2 →