LIPUS inhibits inflammation and catabolism through the NF-κB pathway in human degenerative nucleus pulposus cells

被引：12

作者：

Yi, Weiwei ^{[1
]}

Chen, Qing ^{[1
]}

Liu, Chuan ^{[2
]}

Li, Kaiting ^{[1
]}

Tao, Bailong ^{[3
]}

Tian, Guihua ^{[1
]}

Zhou, Lu ^{[1
]}

Li, Xiaohong ^{[1
]}

Shen, Jieliang ^{[4
]}

Liu, Bo ^{[4
]}

Hu, Zhenming ^{[4
]}

Wang, Dawu ^{[1
]}

Bai, Dingqun ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 1, Dept Rehabil Med, 1 Shiyou St, Chongqing 400010, Peoples R China

[2] Chongqing Med Univ, Lab Res Ctr, Affiliated Hosp 1, 1 Shiyou St, Chongqing 400010, Peoples R China

[3] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 1, 1 Shiyou St, Chongqing 400010, Peoples R China

[4] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, 1 Shiyou St, Chongqing 400010, Peoples R China

来源：

JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH | 2021年 / 16卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Low-intensity pulsed ultrasound; NF-kappa B; Inflammation; Nucleus pulposus cells; INTENSITY PULSED ULTRASOUND; DISC DEGENERATION; DEGRADATION; EXPRESSION;

D O I：

10.1186/s13018-021-02739-1

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Background: Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods: Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm(2), 30 mW/cm(2) and 60 mW/cm(2)) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-alpha and IL-1 beta. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real-time PCR and western blotting, respectively. The activity of the nuclear factor-kappa B (NF-kappa B) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-kappa B inhibitor PDTC, the expression of TNF-alpha, IL-1 beta, MMP-3 and MMP-9 was measured by real-time PCR. Results: LIPUS at intensities of 15 mW/cm(2), 30 mW/cm(2) and 60 mW/cm(2) inhibited LPS-induced NP cell expression of the inflammatory factors TNF-alpha and IL-1 beta, especially at 30 mW/cm(2). LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF-kappa B signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-kappa B inhibitor PDTC by inhibiting the NF-kappa B signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells. Conclusion: LIPUS inhibited inflammation and catabolism through the NF-kappa B pathway in human degenerative nucleus pulposus cells.

引用

页数：9

共 29 条

[21] TNF-α and IL-1β Promote a Disintegrin-like and Metalloprotease with Thrombospondin Type I Motif-5-mediated Aggrecan Degradation through Syndecan-4 in Intervertebral Disc [J].