The first transcriptome dataset of roselle ( Hibiscus sabdariffa L. ) calyces during maturation

Roselle ( Hibiscus sabdariffa L.) is recognized for its phyto-chemical compounds such as anthocyanins, which possess pharmacological potentials in the treatments of hyperten-sion, diabetes, cancer, hyperlipidaemia and hyperglycaemia. The calyx is the most commercially valuable part of the roselle and usually harvested at maturation. However, genetic study to understand the transcriptome changes in the ca-lyx during maturation has yet to be explored. In this study, we sequenced the transcriptomes of roselle calyces at mat-uration stages III and IV using Illumina NextSeq 500 plat-form. These are the two most critical maturation stages in roselle, as these stages are often associated with the qual-ity of the calyx. Over 200 million good quality paired-end reads were generated and de novo assembled into a ref-erence transcriptome consisting of 221,334 transcripts with N50 score of 491bp. Among these transcripts, 92,974 tran-scripts (42%) were successfully annotated. The total number of significantly differentially expressed genes (DEGs) and the top five most significantly regulated genes in each of the maturation stage were presented. Twenty-one genes impli-cated in the biosynthesis of anthocyanins and their relative expressions in the calyx tissues at the two maturation stages were reported. Two secondary metabolites biosynthesis path-ways that attained a relatively higher number of DEG map-pings compared to other pathways were also reported. The findings from this work provide novel insights to better un-derstand the transcriptional changes in roselle during calyx maturation, and the data made available here is intended for continued genetic study on roselle. The work is registered under NCBI Bioproject PRJNA664826. The raw sequencing reads are available in Short Read Archive with the accession numbers SRX9171161, SRX9171162, SRX9171163, SRX9171164, SRX9171165 and SRX9171166.(c) 2022 The Authors. Published by Elsevier Inc.This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )